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PRJNA565268 SRP221525

Gut metagenome, healthy adults, Shanghai, tea intervention

gut metagenome

2 gut metagenome samples from healthy adult controls in Shanghai, China, collected as part of an intervention study examining Pu-erh tea theabrownin's effects on gut microbiota and bile-acid metabolism. machine-written summary

2 samples · 2 runs · CN · Adult · 2019 · 13.69 Gbp / sample

Sources
✓Sequence archive 8○Abstract / description✓Full text 6○Supplementary tables–External resources–User contribution–Human verified

Checked this project's papers and archive records yourself? Mark it human-verified.

what each source gave
✓ Sequence archive8 sample values (collection date, country, location region, timepoint)
○ Abstract / descriptionread; no usable statement
✓ Full text6 sample values (health condition, health condition detail, sex) · PMC6823360
○ Supplementary tablesfiles read; no table could be matched to the samples
– External resourcesnot covered by the external resources we ingest
– User contributionnothing uploaded yet
– Human verifiednot yet checked by a person · I checked this project — verify it
Samples at a glance
1 country
Age
adult2
Condition
healthy_control2
Sex
male2
Country
CN2
Body site
primary2
Sequencing depth per sample · this project all projects
all projects, 0–0.03 Gbp: 25,080 samples (4 %)all projects, 0.03–0.1 Gbp: 17,066 samples (3 %)all projects, 0.1–0.3 Gbp: 29,179 samples (5 %)all projects, 0.3–1 Gbp: 47,044 samples (8 %)all projects, 1–3 Gbp: 124,983 samples (22 %)all projects, 3–10 Gbp: 253,728 samples (44 %)all projects, 10–30 Gbp: 72,533 samples (13 %)this project, 10–30 Gbp: 2 samplesall projects, 30–100 Gbp: 3,024 samples (1 %)all projects, 100–300 Gbp: 106 samples (0 %)all projects, ≥ 300 Gbp: 9 samples (0 %)project median 13.69 Gbp per sample0130Gbp
Community composition
Faecalibacterium 16.2%Roseburia 14.0%Prevotella 13.8%Ruminococcoides 4.5%Dialister 4.1%Gemmiger 3.7%Megamonas 3.2%Phocaeicola 2.5%Bacteroides 2.0%Blautia_A 1.6%Bifidobacterium 1.5%Coprococcus_C 1.4%not assigned to a genus 3.5%other genera 27.9%

Genera, mean relative abundance over 2 profiled gut samples of this project (Sandpiper SingleM profiles, GTDB R232; low-depth and non-metagenome profiles left out).

Field coverage
fieldsamplescoverageR1R2R3R4
age_category2 100%
country2 100%2
health_condition2 100%2
subject_id0 0%
collection_date2 100%2
detailed_location2 100%
health_condition_detail2 100%2
location_region2 100%2
sex2 100%2
timepoint_label2 100%2
Papers (1)
  • Theabrownin from Pu-erh tea attenuates hypercholesterolemia via modulation of gut microbiota and bile acid metabolism. 2019 PMID 31672964 PMC6823360 own_data
Details
Archive description

metagenom shotgun sequencing, human gut

How this project entered the registry

Classified as human gut / stool shotgun DNA metagenome of adult by deterministic_prior with confidence 0.90. Assay shotgun_dna.

  • host external_curation.infant_catalog · “infant triage reason_code age_adult_only”
  • body site study.title · “gut metagenome”
  • body site study.description · “shotgun sequencing, human gut”
  • life stage external_curation.infant_catalog · “infant triage reason_code age_adult_only”
Evidence examples (one per field × route)
samplefieldvaluerouteconf.sourcequote
SAMN12739624collection_date2017-02-03R10.90biosample.attribute:collection_date2017-02-03T08:15:00Z
SAMN12739624countryCNR10.90sample.attr.geo_loc_nameChina:Shanghai
SAMN12739624health_conditionhealthy_controlR30.70paper.fulltext.methodsThirteen healthy male volunteers were enrolled in this study
SAMN12739624health_condition_detailhealthy male volunteersR30.70paper.fulltext.methodsThirteen healthy male volunteers were enrolled in this study
SAMN12739624location_regionShanghaiR10.85biosample.attribute:geo_loc_nameChina:Shanghai
SAMN12739624sexmaleR30.70paper.fulltext.methodsThirteen healthy male volunteers were enrolled in this study
SAMN12739624timepoint_labelPre-PTeaR10.70sample.name_rule:aliasPre-PTea
Sequencing

2 runs over 2 samples · mean 13.69 Gbp per run (median 13.69 Gbp, total 27.37 Gbp; 2 runs with a base count) · layout PAIRED 2 · platform ILLUMINA 2 · models HiSeq X Ten (2) · 100% of runs with a Sandpiper profile · first public 2019-09-13

Authors (27)

Huang F first · Zheng X · Ma X · Jiang R · Zhou W · Zhou S · Zhang Y · Lei S · Wang S · Kuang J · Han X · Wei M · You Y · Li M · Li Y · Liang D · Liu J · Chen T · Yan C · Wei R · Rajani C · Shen C · Xie G · Bian Z · Li H · Zhao A · Jia W last

Downloads

PRJNA565268.csv.gz (950 B) · parquet (69.6 KB) — all 2 samples, every field with route and confidence · PRJNA565268_determinations.csv.gz (820 B) — 14 evidence rows

Samples (2 · every field this project has values for · hover a cell for its source)
Loading the sample table…