Crossover trial of FOS/PDX prebiotics in healthy US adults
491 stool samples from healthy adult volunteers in Illinois, USA, in a randomized, double-blind, two-cohort, two-period crossover study comparing gut microbiome effects of oligofructose (FOS) and polydextrose (PDX) intake. machine-written summary
491 samples · 491 runs · US · Adult · 2020 · 0.14 Gbp / sample · cohort High-resolution temporal profiling of the human gu
Checked this project's papers and archive records yourself? Mark it human-verified.
what each source gave
| ✓ Sequence archive | 4,910 sample values (collection date, country, health condition, intervention, intervention detail, latitude, location region, longitude) |
| ✓ Abstract / description | 491 sample values (health condition detail) |
| ○ Full text | read; no usable statement or table · PMC7386241 |
| – Supplementary tables | no supplementary tables available (or none open access) |
| – External resources | not covered by the external resources we ingest |
| – User contribution | nothing uploaded yet |
| – Human verified | not yet checked by a person · I checked this project — verify it |
intervention · arms resolved
Prebiotic / dietary fibre supplement design crossover · population healthy_control · FOS (oligofructose) and PDX (polydextrose) given in crossover order, randomized dose
“Compare the Effect of Oligofructose (FOS) and Polydextrose (PDX)” — bioproject.description (study level; per-sample arms are in the intervention column below)
longitudinal
10 of 10 people sampled more than once (491 samples; median 58, up to 65 per person) · follow-up median 3 d (max 2 mo) · median interval between samples 3 d (time from collection dates). Schedule stated by the source: “Repeated sampling across Run-In, Feeding1, Washout1, Feeding2, Washout2 periods per subject”.
Each row is one person (3 shown of 10); dots are samples at days since that person's first sample.
Genera, mean relative abundance over 392 profiled gut samples of this project (Sandpiper SingleM profiles, GTDB R232; low-depth and non-metagenome profiles left out).
| field | samples | coverage | R1 | R2 | R3 | R4 |
|---|---|---|---|---|---|---|
| age_category | 491 | 100% | ||||
| country | 491 | 100% | 491 | |||
| health_condition | 491 | 100% | 491 | |||
| subject_id | 491 | 100% | 491 | |||
| collection_date | 491 | 100% | 491 | |||
| detailed_location | 491 | 100% | ||||
| health_condition_detail | 491 | 100% | 491 | |||
| intervention | 491 | 100% | 491 | |||
| intervention_detail | 491 | 100% | 491 | |||
| latitude | 491 | 100% | 491 | |||
| location_region | 491 | 100% | 491 | |||
| longitude | 491 | 100% | 491 | |||
| timepoint_label | 491 | 100% | 491 |
- High-resolution temporal profiling of the human gut microbiome reveals consistent and cascading alterations in response to dietary glycans. 2020 PMID 32620143 PMC7386241 unsure
Archive description
A Randomized, Double-Blind, Two-Cohort, Two-Period, Crossover Study to Compare the Effect of Oligofructose (FOS) and Polydextrose (PDX) on Gut Microbiota Structure and Function in Healthy Subjects
How this project entered the registry
Classified as human gut / stool shotgun DNA metagenome of adult by deterministic_prior with confidence 0.90. Assay shotgun_dna.
- host external_curation.infant_catalog · “infant triage reason_code age_adult_only”
- body site study.title · “Human gut microbiome response to”
- body site study.description · “Polydextrose (PDX) on Gut Microbiota Structure and”
- life stage external_curation.infant_catalog · “infant triage reason_code age_adult_only”
Evidence examples (one per field × route)
| sample | field | value | route | conf. | source | quote |
|---|---|---|---|---|---|---|
| SAMN13522442 | collection_date | 2017-07-21 | R1 | 0.90 | biosample.attribute:collection_date | 2017-07-21 |
| SAMN13522442 | country | US | R1 | 0.90 | sample.attr.geo_loc_name | USA: Illinois |
| SAMN13522442 | health_condition | healthy_control | R1 | 0.70 | biosample_attr | FOS->PDX |
| SAMN13522442 | health_condition_detail | healthy subjects | R4 | 0.50 | study.description | healthy Subjects |
| SAMN13522442 | intervention | prebiotic_or_fibre | R1 | 0.80 | biosample.attribute:period | period: Feeding1 |
| SAMN13522442 | intervention_detail | FOS feeding period (first arm of crossover) | R1 | 0.80 | biosample.attribute:period | period: Feeding1 |
| SAMN13522442 | latitude | 41.0000 | R1 | 0.90 | biosample.attribute:lat_lon | 41 N 88 W |
| SAMN13522442 | location_region | Illinois | R1 | 0.85 | biosample.attribute:geo_loc_name | USA: Illinois |
| SAMN13522442 | longitude | -88.0000 | R1 | 0.90 | biosample.attribute:lat_lon | 41 N 88 W |
| SAMN13522442 | subject_id | 301-001 | R1 | 0.85 | sample.attr.subject | 301-001 |
| SAMN13522442 | timepoint_label | Feeding1 | R1 | 0.85 | sample.attr.period | Feeding1 |
Sequencing
491 runs over 491 samples · mean 0.15 Gbp per run (median 0.14 Gbp, total 74.37 Gbp; 491 runs with a base count) · layout SINGLE 491 · platform ILLUMINA 491 · models HiSeq X Ten (491) · 80% of runs with a Sandpiper profile · first public 2020-06-16 – 2020-06-18
Authors (14)
Creswell R first · Tan J · Leff JW · Brooks B · Mahowald MA · Thieroff-Ekerdt R · Gerber GK last · Tarracchini C first · Lugli GA · Mancabelli L · van Sinderen D · Turroni F · Ventura M · Milani C last
Downloads
PRJNA594613.csv.gz (11.0 KB) · parquet (85.6 KB) — all 491 samples, every field with route and confidence · PRJNA594613_determinations.csv.gz (30.6 KB) — 5,401 evidence rows