Crossover trial of FOS vs polydextrose in healthy US adults
561 stool samples from healthy adults in Illinois, USA, in a randomized, double-blind, two-cohort, two-period crossover study comparing gut microbiome effects of oligofructose (FOS) and polydextrose intake. machine-written summary
561 samples · 561 runs · US · Adult · 2020 · 0.13 Gbp / sample · cohort High-resolution temporal profiling of the human gu
Checked this project's papers and archive records yourself? Mark it human-verified.
what each source gave
| ✓ Sequence archive | 5,610 sample values (collection date, country, health condition, intervention, intervention detail, latitude, location region, longitude) |
| ✓ Abstract / description | 561 sample values (health condition detail) |
| – Full text | no open-access full text linked |
| – Supplementary tables | no supplementary tables available (or none open access) |
| – External resources | not covered by the external resources we ingest |
| – User contribution | nothing uploaded yet |
| – Human verified | not yet checked by a person · I checked this project — verify it |
intervention · arms resolved
Prebiotic / dietary fibre supplement design crossover · population healthy_control · Fructooligosaccharides (FOS) and polydextrose (PDX) supplementation, crossover
“Compare the Effect of Oligofructose (FOS) and Polydextrose on Gut Microbiota” — bioproject.description (study level; per-sample arms are in the intervention column below)
longitudinal
10 of 10 people sampled more than once (561 samples; median 62, up to 70 per person) · follow-up median 1 d (max 5 d) · median interval between samples 1 d (time from collection dates). Schedule stated by the source: “Run-In, Feeding1, Washout1, Feeding2, Washout2 periods with repeated sampling per subject” (time origin: intervention start).
Each row is one person (3 shown of 10); dots are samples at days since that person's first sample.
Genera, mean relative abundance over 411 profiled gut samples of this project (Sandpiper SingleM profiles, GTDB R232; low-depth and non-metagenome profiles left out).
| field | samples | coverage | R1 | R2 | R3 | R4 |
|---|---|---|---|---|---|---|
| age_category | 561 | 100% | ||||
| country | 561 | 100% | 561 | |||
| health_condition | 561 | 100% | 561 | |||
| subject_id | 561 | 100% | 561 | |||
| collection_date | 561 | 100% | 561 | |||
| detailed_location | 561 | 100% | ||||
| health_condition_detail | 561 | 100% | 561 | |||
| intervention | 561 | 100% | 561 | |||
| intervention_detail | 561 | 100% | 561 | |||
| latitude | 561 | 100% | 561 | |||
| location_region | 561 | 100% | 561 | |||
| longitude | 561 | 100% | 561 | |||
| timepoint_label | 561 | 100% | 561 |
- High-resolution temporal profiling of the human gut microbiome reveals consistent and cascading alterations in response to dietary glycans. 2020 PMID 32620143 PMC7386241 unsure
Archive description
A Randomized, Double-Blind, Two-Cohort, Two-Period, Crossover Study to Compare the Effect of Oligofructose (FOS) and Polydextrose on Gut Microbiota Structure and Function in Healthy Subjects
How this project entered the registry
Classified as human gut / stool shotgun DNA metagenome of adult by deterministic_prior with confidence 0.90. Assay shotgun_dna.
- host external_curation.infant_catalog · “infant triage reason_code age_adult_only”
- body site study.title · “Human gut microbiome response to”
- body site study.description · “and Polydextrose on Gut Microbiota Structure and”
- life stage external_curation.infant_catalog · “infant triage reason_code age_adult_only”
Evidence examples (one per field × route)
| sample | field | value | route | conf. | source | quote |
|---|---|---|---|---|---|---|
| SAMN13523056 | collection_date | 2017-07-21 | R1 | 0.90 | biosample.attribute:collection_date | 2017-07-21 |
| SAMN13523056 | country | US | R1 | 0.90 | sample.attr.geo_loc_name | USA: Illinois |
| SAMN13523056 | health_condition | healthy_control | R1 | 0.70 | biosample_attr | PDX->FOS |
| SAMN13523056 | health_condition_detail | Healthy volunteers in dietary intervention study | R4 | 0.50 | study.description | healthy Subjects |
| SAMN13523056 | intervention | no_intervention | R1 | 0.90 | biosample.attribute:period | period: Washout1 |
| SAMN13523056 | intervention_detail | washout after PDX feeding period | R1 | 0.90 | biosample.attribute:period | period: Washout1 |
| SAMN13523056 | latitude | 41.0000 | R1 | 0.90 | biosample.attribute:lat_lon | 41 N 88 W |
| SAMN13523056 | location_region | Illinois | R1 | 0.85 | biosample.attribute:geo_loc_name | USA: Illinois |
| SAMN13523056 | longitude | -88.0000 | R1 | 0.90 | biosample.attribute:lat_lon | 41 N 88 W |
| SAMN13523056 | subject_id | 301-010 | R1 | 0.85 | sample.attr.subject | 301-010 |
| SAMN13523056 | timepoint_label | Washout1 | R1 | 0.85 | sample.attr.period | Washout1 |
Sequencing
561 runs over 561 samples · mean 0.14 Gbp per run (median 0.13 Gbp, total 76.74 Gbp; 561 runs with a base count) · layout SINGLE 561 · platform ILLUMINA 561 · models HiSeq X Ten (561) · 73% of runs with a Sandpiper profile · first public 2020-06-16
Authors (14)
Creswell R first · Tan J · Leff JW · Brooks B · Mahowald MA · Thieroff-Ekerdt R · Gerber GK last · Tarracchini C first · Lugli GA · Mancabelli L · van Sinderen D · Turroni F · Ventura M · Milani C last
Downloads
PRJNA594620.csv.gz (12.6 KB) · parquet (87.9 KB) — all 561 samples, every field with route and confidence · PRJNA594620_determinations.csv.gz (35.1 KB) — 6,171 evidence rows