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PRJNA598452 SRP239293

Elderly gut microbiota, diet and stress, Örebro, Sweden

Elderly Gut Microbiota FMM

97 faecal shotgun metagenome samples from senior orienteers and home-dwelling elderly in Örebro, Sweden, studying links between gut microbiota, dietary intake, psychological stress, and gastrointestinal symptoms, in a cross-sectional design. machine-written summary

97 samples · 98 runs · SE · Elderly · 2020 · 12.18 Gbp / sample

Sources
✓Sequence archive 582○Abstract / description○Full text○Supplementary tables–External resources–User contribution–Human verified

Checked this project's papers and archive records yourself? Mark it human-verified.

what each source gave
✓ Sequence archive582 sample values (collection date, country, latitude, location locality, location region, longitude)
○ Abstract / descriptionread; no usable statement
○ Full textread; no usable statement or table · PMC7551621
○ Supplementary tablesfiles read; no table could be matched to the samples
– External resourcesnot covered by the external resources we ingest
– User contributionnothing uploaded yet · share metadata
– Human verifiednot yet checked by a person · I checked this project — verify it
Samples at a glance
1 sampling locations · 1 country
Age
elderly97
Country
SE97
Body site
primary97
Collected 2012–2015
2012: 28 samples2013: 38 samples2015: 31 samples20122015
Sequencing depth per sample · this project all projects
all projects, 0–0.03 Gbp: 25,080 samples (4 %)all projects, 0.03–0.1 Gbp: 17,066 samples (3 %)all projects, 0.1–0.3 Gbp: 29,179 samples (5 %)all projects, 0.3–1 Gbp: 47,044 samples (8 %)all projects, 1–3 Gbp: 124,983 samples (22 %)all projects, 3–10 Gbp: 253,728 samples (44 %)this project, 3–10 Gbp: 4 samplesall projects, 10–30 Gbp: 72,533 samples (13 %)this project, 10–30 Gbp: 93 samplesall projects, 30–100 Gbp: 3,024 samples (1 %)all projects, 100–300 Gbp: 106 samples (0 %)all projects, ≥ 300 Gbp: 9 samples (0 %)project median 12.18 Gbp per sample0130Gbp
Community composition
Bacteroides 6.5%Roseburia 5.6%Faecalibacterium 5.0%Phocaeicola 4.4%Prevotella 3.7%Blautia_A 3.6%Alistipes 3.4%Ruminococcoides 2.8%Gemmiger 1.8%Cryptobacteroides 1.7%Lachnospira 1.6%Bifidobacterium 1.6%not assigned to a genus 3.4%other genera 54.9%

Genera, mean relative abundance over 93 profiled gut samples of this project (Sandpiper SingleM profiles, GTDB R232; low-depth and non-metagenome profiles left out).

Field coverage
fieldsamplescoverageR1R2R3R4
age_category97 100%
country97 100%97
health_condition0 0%
subject_id0 0%
collection_date97 100%97
detailed_location97 100%
latitude97 100%97
location_locality97 100%97
location_region97 100%97
longitude97 100%97
Papers (1)
  • Differences in Gut Microbiome Composition between Senior Orienteering Athletes and Community-Dwelling Older Adults. 2020 PMID 32867153 PMC7551621 own_data
Details
Archive description

This project is aimed to understand the influence of diet and psychological variables on the gut microbiota of elderly cohorts. Shotgun metagenome sequences were obtained from faecal samples from senior orienteers and home-dwelling elderly, and used for microbiota profiling. These microbiota profiles were used to study the differences among the participating groups based on their dietary intake, psychological stress, and gastrointestinal symptoms rating scales.

How this project entered the registry

Classified as human gut / stool shotgun DNA metagenome of elderly by opus_adjudicated with confidence 0.70. Assay shotgun_dna.

  • host run.scientific_name · “human gut metagenome”
  • host study.description · “home-dwelling elderly”
  • body site study.description · “obtained from faecal samples”
  • life stage study.description · “senior orienteers and home-dwelling elderly”
Evidence examples (one per field × route)
samplefieldvaluerouteconf.sourcequote
SAMN13705497collection_date2015-09R10.90biosample.attribute:collection_date2015-09
SAMN13705497countrySER10.90sample.attr.geo_loc_nameSweden: Orebro
SAMN13705497latitude59.2753R10.90biosample.attribute:lat_lon59.2753 N 15.2134 E
SAMN13705497location_localityÖrebroR10.85biosample.attribute:geo_loc_nameSweden: Orebro
SAMN13705497location_regionOErebroR10.60biosample.attribute:lat_lon59.2753 N 15.2134 E
SAMN13705497longitude15.2134R10.90biosample.attribute:lat_lon59.2753 N 15.2134 E
Sequencing

98 runs over 97 samples · mean 12.64 Gbp per run (median 12.13 Gbp, total 1,238.45 Gbp; 98 runs with a base count) · layout PAIRED 98 · platform ILLUMINA 98 · models Illumina HiSeq 2500 (98) · 95% of runs with a Sandpiper profile · first public 2020-02-07 – 2020-02-09

Authors (0)

No author record.

Help complete this project

2 of the 4 core fields are below 50% coverage: health_condition, subject_id. Share metadata

Downloads

PRJNA598452.csv.gz (3.2 KB) · parquet (73.4 KB) — all 97 samples, every field with route and confidence · PRJNA598452_determinations.csv.gz (3.7 KB) — 582 evidence rows

Samples (97 · every field this project has values for · hover a cell for its source)
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