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PRJNA637785 SRP266254

Multi-donor FMT trial in obese adolescents, Auckland NZ

Multi-donor FMT to treat adolescents with severe obesity (The Gut Bugs Trial)

429 stool samples from adolescents with severe obesity and their FMT donors in Auckland, New Zealand, from a randomized, double-blind, placebo-controlled trial of lean multi-donor fecal microbiota transplantation versus placebo. machine-written summary

430 samples · 430 runs · NZ · Child · 2021 · 5.97 Gbp / sample

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Sources
✓Sequence archive 4,345✓Abstract / description 381✓Full text 2○Supplementary tables–External resources–User contribution–Human verified

Checked this project's papers and archive records yourself? Mark it human-verified.

what each source gave
✓ Sequence archive4,345 sample values (age, collection date, country, health condition, health condition detail, intervention, intervention detail, location locality)
✓ Abstract / description381 sample values (collection date)
✓ Full text2 sample values (health condition, health condition detail) · PMC10860221, PMC11227244, PMC12180181, PMC12394558, PMC12710892, PMC7753902, PMC8120839
○ Supplementary tablesfiles read; no table could be matched to the samples
– External resourcesnot covered by the external resources we ingest
– User contributionnothing uploaded yet
– Human verifiednot yet checked by a person · I checked this project — verify it
Interventions

intervention · arms resolved

RFaecal microbiota transplantation: 41 people · 135 samplesFMT 41 people · 135 samplesPlacebo / sham arm (sample level only): 44 people · 150 samplesplacebo 44 people · 150 samplesControl / pre-intervention baseline (sample level only): 96 people · 145 samplesno intervention 96 people · 145 samples
randomised to parallel arms

85 people have samples in more than one arm.

Bowel cleansing / colonoscopy preparation Faecal microbiota transplantation design randomized_controlled_trial · population obesity · Multi-donor oral encapsulated FMT from 4 lean donors vs placebo

“Single course of oral encapsulated fecal microbiome from 4 healthy lean donors” — abstract:33346848 (study level; per-sample arms are in the intervention column below)

Samples at a glance
1 country
Age
adult244
adolescent186
Condition
obesity372
healthy_control58
Sex
female251
male179
Country
NZ430
Body site
primary430
Collected 2017–2023
2017: 381 samples2022: 40 samples2023: 9 samples20172023
Sequencing depth per sample · this project all projects
all projects, 0–0.03 Gbp: 25,080 samples (4 %)all projects, 0.03–0.1 Gbp: 17,066 samples (3 %)all projects, 0.1–0.3 Gbp: 29,179 samples (5 %)all projects, 0.3–1 Gbp: 47,044 samples (8 %)all projects, 1–3 Gbp: 124,983 samples (22 %)this project, 1–3 Gbp: 1 samplesall projects, 3–10 Gbp: 253,728 samples (44 %)this project, 3–10 Gbp: 429 samplesall projects, 10–30 Gbp: 72,533 samples (13 %)all projects, 30–100 Gbp: 3,024 samples (1 %)all projects, 100–300 Gbp: 106 samples (0 %)all projects, ≥ 300 Gbp: 9 samples (0 %)project median 5.97 Gbp per sample0130Gbp
Sampling design and relatedness

longitudinalFMT donor → recipient

93 of 96 people sampled more than once (427 samples; median 5, up to 9 per person) · follow-up median 4.0 y (max 5.0 y) · median interval between samples 12 mo (time from age at collection). Schedule stated by the source: “Recipients: baseline, 6, 12, 26 weeks post-treatment, plus a subset at 4-year follow-up; donors: multiple donation timepoints” (time origin: FMT treatment/intervention start); stated follow-up 4.0 y.

TF02TF02: day 0TF02: day 0TF02: day 0TF02: day 0TF02: day 1826TF05TF05: day 0TF05: day 0TF05: day 0TF05: day 0TF05: day 1816TF07TF07: day 0TF07: day 0TF07: day 369TF07: day 369TF07: day 1830TF10TF10: day 0TF10: day 0TF10: day 364TF10: day 364TF10: day 1816TF15TF15: day 0TF15: day 0TF15: day 0TF15: day 0TF15: day 1461TF18TF18: day 0TF18: day 0TF18: day 0TF18: day 0TF18: day 1830TF19TF19: day 0TF19: day 364TF19: day 364TF19: day 364TF19: day 1816TF21TF21: day 0TF21: day 0TF21: day 0TF21: day 0TF21: day 1461TF22TF22: day 0TF22: day 0TF22: day 0TF22: day 0TF22: day 1452TF26TF26: day 0TF26: day 364TF26: day 364TF26: day 364TF26: day 1816TF27TF27: day 0TF27: day 0TF27: day 0TF27: day 0TF27: day 1452TF28TF28: day 0TF28: day 0TF28: day 0TF28: day 0TF28: day 1461TF29TF29: day 0TF29: day 0TF29: day 0TF29: day 0TF29: day 1461TF32TF32: day 0TF32: day 0TF32: day 369TF32: day 369TF32: day 1830TF34TF34: day 0TF34: day 0TF34: day 0TF34: day 0TF34: day 1461TF35TF35: day 0TF35: day 0TF35: day 0TF35: day 0TF35: day 1464TF36TF36: day 0TF36: day 0TF36: day 0TF36: day 365TF36: day 1460TF37TF37: day 0TF37: day 0TF37: day 0TF37: day 0TF37: day 1464TF38TF38: day 0TF38: day 0TF38: day 0TF38: day 366TF38: day 1461TF43TF43: day 0TF43: day 369TF43: day 369TF43: day 369TF43: day 1830TF45TF45: day 0TF45: day 0TF45: day 0TF45: day 0TF45: day 1464TF47TF47: day 0TF47: day 0TF47: day 0TF47: day 369TF47: day 1464TF49TF49: day 0TF49: day 365TF49: day 365TF49: day 365TF49: day 1826TF51TF51: day 0TF51: day 0TF51: day 0TF51: day 0TF51: day 1460TM03TM03: day 0TM03: day 0TM03: day 0TM03: day 369TM03: day 1464TM06TM06: day 0TM06: day 0TM06: day 0TM06: day 0TM06: day 1452TM07TM07: day 0TM07: day 0TM07: day 369TM07: day 369TM07: day 1830TM08TM08: day 0TM08: day 0TM08: day 0TM08: day 0TM08: day 1827TM09TM09: day 0TM09: day 0TM09: day 0TM09: day 0TM09: day 1461TM12TM12: day 0TM12: day 0TM12: day 0TM12: day 0TM12: day 1816TM16TM16: day 0TM16: day 0TM16: day 0TM16: day 0TM16: day 1464TM17TM17: day 0TM17: day 365TM17: day 365TM17: day 365TM17: day 1826TM18TM18: day 0TM18: day 0TM18: day 366TM18: day 366TM18: day 1826TM20TM20: day 0TM20: day 0TM20: day 0TM20: day 365TM20: day 1460TM21TM21: day 0TM21: day 0TM21: day 0TM21: day 364TM21: day 1461TM23TM23: day 0TM23: day 0TM23: day 0TM23: day 0TM23: day 14610 d15 mo2.5 y3.8 y5.0 y

Each row is one person (36 shown of 93); dots are samples at days since that person's first sample.

FMT trial: 42 FMT recipients linked by treatment batch to 4 sex-matched lean donors each (9 donor subjects sampled per donation); 45 placebo recipients.

donor: DF12, DF14, DF16, DF17, TF01, TF03, TF04, TF08, TF10, TF11, TF13, TF16, TF19, TF20, TF21, TF22, TF23, TF28, TF29, TF30, TF34, TF35, TF36, TF38, TF39, TF42, TF43, TF46, TF49DF12 donor of TF01DF14 donor of TF01DF16 donor of TF01DF17 donor of TF01DF12 donor of TF03DF14 donor of TF03DF16 donor of TF03DF17 donor of TF03DF12 donor of TF04DF14 donor of TF04DF16 donor of TF04DF17 donor of TF04DF12 donor of TF08DF14 donor of TF08DF16 donor of TF08DF17 donor of TF08DF12 donor of TF10DF14 donor of TF10DF16 donor of TF10DF17 donor of TF10DF12 donor of TF11DF14 donor of TF11DF16 donor of TF11DF17 donor of TF11DF12 donor of TF13DF14 donor of TF13DF16 donor of TF13DF17 donor of TF13DF12 donor of TF16DF14 donor of TF16DF16 donor of TF16DF17 donor of TF16DF12 donor of TF19DF14 donor of TF19DF16 donor of TF19DF17 donor of TF19DF12 donor of TF20DF14 donor of TF20DF16 donor of TF20DF17 donor of TF20DF12 donor of TF21DF14 donor of TF21DF16 donor of TF21DF17 donor of TF21DF12 donor of TF22DF14 donor of TF22DF16 donor of TF22DF17 donor of TF22DF12 donor of TF23DF14 donor of TF23DF16 donor of TF23DF17 donor of TF23DF12 donor of TF28DF14 donor of TF28DF16 donor of TF28DF17 donor of TF28DF12 donor of TF29DF14 donor of TF29DF16 donor of TF29DF17 donor of TF29DF12 donor of TF30DF14 donor of TF30DF16 donor of TF30DF17 donor of TF30DF12 donor of TF34DF14 donor of TF34DF16 donor of TF34DF17 donor of TF34DF12 donor of TF35DF14 donor of TF35DF16 donor of TF35DF17 donor of TF35DF12 donor of TF36DF14 donor of TF36DF16 donor of TF36DF17 donor of TF36DF12 donor of TF38DF14 donor of TF38DF16 donor of TF38DF17 donor of TF38DF12 donor of TF39DF14 donor of TF39DF16 donor of TF39DF17 donor of TF39DF12 donor of TF42DF14 donor of TF42DF16 donor of TF42DF17 donor of TF42DF12 donor of TF43DF14 donor of TF43DF16 donor of TF43DF17 donor of TF43DF12 donor of TF46DF14 donor of TF46DF16 donor of TF46DF17 donor of TF46DF12 donor of TF49DF14 donor of TF49DF16 donor of TF49DF17 donor of TF49DF12 — donor · 8 samplesDDF12DF14 — donor · 8 samplesDDF14DF16 — donor · 9 samplesDDF16DF17 — donor · 9 samplesDDF17TF01 — recipient · 4 samplesRTF01TF03 — recipient · 4 samplesRTF03TF04 — recipient · 1 sampleRTF04TF08 — recipient · 2 samplesRTF08TF10 — recipient · 5 samplesRTF10TF11 — recipient · 4 samplesRTF11TF13 — recipient · 4 samplesRTF13TF16 — recipient · 4 samplesRTF16TF19 — recipient · 5 samplesRTF19TF20 — recipient · 4 samplesRTF20TF21 — recipient · 5 samplesRTF21TF22 — recipient · 5 samplesRTF22TF23 — recipient · 4 samplesRTF23TF28 — recipient · 5 samplesRTF28TF29 — recipient · 5 samplesRTF29TF30 — recipient · 4 samplesRTF30TF34 — recipient · 5 samplesRTF34TF35 — recipient · 5 samplesRTF35TF36 — recipient · 5 samplesRTF36TF38 — recipient · 5 samplesRTF38TF39 — recipient · 4 samplesRTF39TF42 — recipient · 4 samplesRTF42TF43 — recipient · 5 samplesRTF43TF46 — recipient · 3 samplesRTF46TF49 — recipient · 5 samplesRTF49
donor: DM03, DM05, DM07, DM08, DM12, TM01, TM02, TM04, TM05, TM08, TM10, TM11, TM14, TM17, TM18, TM19, TM21, TM28, TM29, TM30, TM34, TM35DM03 donor of TM01DM05 donor of TM01DM07 donor of TM01DM08 donor of TM01DM03 donor of TM02DM05 donor of TM02DM07 donor of TM02DM08 donor of TM02DM03 donor of TM04DM05 donor of TM04DM07 donor of TM04DM08 donor of TM04DM03 donor of TM05DM05 donor of TM05DM07 donor of TM05DM08 donor of TM05DM03 donor of TM08DM05 donor of TM08DM07 donor of TM08DM08 donor of TM08DM03 donor of TM10DM07 donor of TM10DM08 donor of TM10DM12 donor of TM10DM03 donor of TM11DM05 donor of TM11DM07 donor of TM11DM08 donor of TM11DM03 donor of TM14DM07 donor of TM14DM08 donor of TM14DM12 donor of TM14DM03 donor of TM17DM07 donor of TM17DM08 donor of TM17DM12 donor of TM17DM03 donor of TM18DM07 donor of TM18DM08 donor of TM18DM12 donor of TM18DM03 donor of TM19DM07 donor of TM19DM08 donor of TM19DM12 donor of TM19DM03 donor of TM21DM07 donor of TM21DM08 donor of TM21DM12 donor of TM21DM03 donor of TM28DM07 donor of TM28DM08 donor of TM28DM12 donor of TM28DM03 donor of TM29DM07 donor of TM29DM08 donor of TM29DM12 donor of TM29DM03 donor of TM30DM07 donor of TM30DM08 donor of TM30DM12 donor of TM30DM03 donor of TM34DM07 donor of TM34DM08 donor of TM34DM12 donor of TM34DM03 donor of TM35DM07 donor of TM35DM08 donor of TM35DM12 donor of TM35DM03 — donor · 6 samplesDDM03DM05 — donor · 1 sampleDDM05DM07 — donor · 6 samplesDDM07DM08 — donor · 6 samplesDDM08DM12 — donor · 5 samplesDDM12TM01 — recipient · 4 samplesRTM01TM02 — recipient · 4 samplesRTM02TM04 — recipient · 4 samplesRTM04TM05 — recipient · 4 samplesRTM05TM08 — recipient · 5 samplesRTM08TM10 — recipient · 4 samplesRTM10TM11 — recipient · 2 samplesRTM11TM14 — recipient · 4 samplesRTM14TM17 — recipient · 5 samplesRTM17TM18 — recipient · 5 samplesRTM18TM19 — recipient · 2 samplesRTM19TM21 — recipient · 5 samplesRTM21TM28 — recipient · 5 samplesRTM28TM29 — recipient · 5 samplesRTM29TM30 — recipient · 5 samplesRTM30TM34 — recipient · 5 samplesRTM34TM35 — recipient · 3 samplesRTM35

donorrecipient · circles are people (pale = no sequenced sample), arrows run donor → recipient and parent → child, curved lines join twins, partners and siblings, squares are shared settings or families without recorded roles. Shown: 2 linked groups from explicit relations. 96 people in the project overall.

How longitudinal and relatedness information is derived

Community composition
Prevotella 12.8%Roseburia 12.6%Faecalibacterium 9.2%Bacteroides 5.5%Phocaeicola 4.6%Alistipes 2.7%Bifidobacterium 2.2%Ruminococcoides 2.2%Fusicatenibacter 2.1%Dialister 2.0%Blautia_A 1.6%Dorea_A 1.5%not assigned to a genus 4.3%other genera 36.7%

Genera, mean relative abundance over 397 profiled gut samples of this project (Sandpiper SingleM profiles, GTDB R232; low-depth and non-metagenome profiles left out).

Field coverage
fieldsamplescoverageR1R2R3R4
age_category430 100%
country430 100%430
health_condition430 100%4291
subject_id430 100%430
collection_date430 100%49381
detailed_location430 100%
health_condition_detail430 100%4291
intervention430 100%430
location_locality430 100%430
sex430 100%430
timepoint_label430 100%430
age_at_collection_days429 100%429
intervention_detail429 100%429
Papers (7)
  • Strain engraftment competition and functional augmentation in a multi-donor fecal microbiota transplantation trial for obesity. 2021 PMID 33985595 PMC8120839 own_data
  • Horizontal gene transfer after faecal microbiota transplantation in adolescents with obesity. 2024 PMID 38347627 PMC10860221 own_data
  • Fecal microbiota transplantation alters gut phage communities in a clinical trial for obesity. 2024 PMID 38970126 PMC11227244 own_data
  • Long-term health outcomes in adolescents with obesity treated with faecal microbiota transplantation: 4-year follow-up. 2025 PMID 40877311 PMC12394558 own_data
  • Effects of Fecal Microbiome Transfer in Adolescents With Obesity 2020 PMID 33346848 PMC7753902 own_data
2 more
  • Engrafting gut bacteriophages have potential to modulate microbial metabolism in fecal microbiota transplantation. 2025 PMID 40542451 PMC12180181
  • Cohort-specific determinants of donor strain engraftment following multi-donor faecal microbiota transplantation in two randomised clinical trials. 2025 PMID 41378445 PMC12710892
Details
Archive description

The gut microbiome is a contributing factor in the development of metabolic disorders. The overarching goal of this study was to assess the efficacy of using lean multi-donor FMT to treat adolescents with severe obesity. The Gut Bugs Trial recruited 87 adolescents with severe obesity and randomised them 1:1 to receive a single dose of either FMT or placebo capsules. FMT capsules contained concentrated fecal material derived from 4 sex-matched lean donors. Placebo capsules contained saline. Recipients were clinically assessed at baseline, and at 6-, 12-, and 26-weeks post-treatment. The primary objective was a change in BMI SDS at week 6. Secondary objectives included a variety of metabolic health parameters, body composition, and gut microbiome alterations.

How this project entered the registry

Classified as human gut / stool shotgun DNA metagenome of adolescent and child and adult by deterministic_prior with confidence 0.90. Assay shotgun_dna.

  • host external_curation.infant_catalog · “infant triage reason_code age_child_over_36m”
  • body site study.title · “severe obesity (The Gut Bugs Trial)”
  • body site study.description · “The gut microbiome is a”
  • life stage study.title · “FMT to treat adolescents with severe obesity”
  • life stage study.description · “FMT to treat adolescents with severe obesity.”
  • life stage external_curation.infant_catalog · “infant triage reason_code age_child_over_36m”
  • life stage sample.attr.host_age · “18 (n=244/430)”
Evidence examples (one per field × route)
samplefieldvaluerouteconf.sourcequote
SAMN15156364age_at_collection_days6939.0R10.85sample.attr.host_age19
SAMN15156679age_at_collection_days5113.5R10.70biosample.attribute:host_age14
SAMN44563938collection_date2023-01-12R10.90biosample.attribute:collection_date2023-01-12
SAMN15156364collection_date2017-10/2019-03R40.50paper.abstractOctober 2017-March 2019
SAMN15156364countryNZR10.90sample.attr.geo_loc_nameNew Zealand: Auckland
SAMN15156364health_conditionhealthy_controlR10.85biosample.attribute:participant_typedonor
SAMN15156422health_conditionobesityR10.85biosample_attrFMT
SAMN15156679health_conditionobesityR30.60paper.fulltext.resultsimpact of FMT on adolescent obesity
SAMN15156364health_condition_detaildonorR10.85biosample.attribute:participant_typedonor
SAMN15156679health_condition_detailsevere obesity in adolescentsR30.60paper.fulltext.resultsimpact of FMT on adolescent obesity
SAMN15156364interventionno_interventionR10.90biosample.attribute:timepointtimepoint: donation 2
SAMN15156422interventionfmtR10.90biosample.attribute:treatment_grouptreatment_group: FMT
SAMN15156364intervention_detaildonor stool donation sampleR10.90biosample.attribute:timepointtimepoint: donation 2
SAMN15156422intervention_detailFMT capsule armR10.90biosample.attribute:treatment_grouptreatment_group: FMT
SAMN15156364location_localityAucklandR10.85biosample.attribute:geo_loc_nameNew Zealand: Auckland
SAMN15156364sexfemaleR10.90sample.attr.host_sexfemale
SAMN15156364subject_idDF12R10.85sample.attr.host_subject_idDF12
SAMN15156679subject_idTM18R10.85biosample.attribute:host_subject_idTM18
SAMN15156364timepoint_labeldonation 2R10.85sample.attr.timepointdonation 2
SAMN15156679timepoint_labelweek 6R10.85biosample.attribute:timepointweek 6
Sequencing

430 runs over 430 samples · mean 6.06 Gbp per run (median 5.97 Gbp, total 2,604.57 Gbp; 430 runs with a base count) · layout PAIRED 430 · platform ILLUMINA 430 · models Illumina NovaSeq 6000 (430) · 93% of runs with a Sandpiper profile · first public 2021-05-15 – 2025-12-25

Authors (23)

Leong KSW first · Jayasinghe TN · Wilson BC · Derraik JGB · Albert BB · Chiavaroli V · Svirskis DM · Beck KL · Conlon CA · Jiang Y · Schierding W · Vatanen T · Holland DJ · O'Sullivan JM · Cutfield WS last · Behling AH first · Ho D · Zuppi M first · Golovina E · Portlock T · Vickers MH · Tweedie-Cullen RY · Gut Bugs Study Group last

Downloads

PRJNA637785.csv.gz (13.0 KB) · parquet (87.5 KB) — all 430 samples, every field with route and confidence · PRJNA637785_determinations.csv.gz (26.7 KB) — 4,728 evidence rows

Samples (430 · every field this project has values for · hover a cell for its source)
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