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PRJNA662107 SRP320183

Gut/oral microbiome, ethnicity and diet, healthy US females

human metagenome Raw sequence reads

522 gut and oral metagenome samples from healthy Black and White adult female subjects in the USA, studying ethnicity-associated microbiome variation with a controlled vegetarian diet intervention, in a longitudinal design. machine-written summary

522 samples · 522 runs · US · Age unknown · 2021 · 2.29 Gbp / sample

Sources
✓Sequence archive 3,366○Abstract / description✓Full text 2,610○Supplementary tables–External resources–User contribution–Human verified

Checked this project's papers and archive records yourself? Mark it human-verified.

what each source gave
✓ Sequence archive3,366 sample values (collection date, country, latitude, location region, longitude, subject id, timepoint)
○ Abstract / descriptionread; no usable statement
✓ Full text2,610 sample values (health condition, health condition detail, location locality, location site, sex) · PMC11986028, PMC9397868
○ Supplementary tablesfiles read; no table could be matched to the samples
– External resourcesnot covered by the external resources we ingest
– User contributionnothing uploaded yet
– Human verifiednot yet checked by a person · I checked this project — verify it
Interventions

intervention — arms not resolved

Controlled / prescribed diet or feeding regimen design single_arm_before_after · population healthy_control · 4-day controlled vegetarian diet intervention

“a 4-day controlled vegetarian diet intervention” — abstract:35998206 (study level; per-sample arms are in the intervention column below)

Samples at a glance
1 sampling locations · 1 country
Age
adult522
Condition
healthy_control522
Sex
female522
Country
US522
Body site
primary406
excluded71
unknown45
Sequencing depth per sample · this project all projects
all projects, 0–0.03 Gbp: 25,080 samples (4 %)this project, 0–0.03 Gbp: 42 samplesall projects, 0.03–0.1 Gbp: 17,066 samples (3 %)this project, 0.03–0.1 Gbp: 17 samplesall projects, 0.1–0.3 Gbp: 29,179 samples (5 %)this project, 0.1–0.3 Gbp: 23 samplesall projects, 0.3–1 Gbp: 47,044 samples (8 %)this project, 0.3–1 Gbp: 93 samplesall projects, 1–3 Gbp: 124,983 samples (22 %)this project, 1–3 Gbp: 119 samplesall projects, 3–10 Gbp: 253,728 samples (44 %)this project, 3–10 Gbp: 212 samplesall projects, 10–30 Gbp: 72,533 samples (13 %)this project, 10–30 Gbp: 16 samplesall projects, 30–100 Gbp: 3,024 samples (1 %)all projects, 100–300 Gbp: 106 samples (0 %)all projects, ≥ 300 Gbp: 9 samples (0 %)project median 2.29 Gbp per sample0130Gbp
Sampling design and relatedness

longitudinal — not resolved

36 of 222 people sampled more than once (214 samples; median 6, up to 9 per person). Schedule stated by the source: “before, during, and after a 4-day diet intervention” (time origin: intervention_start); stated follow-up 9 d.

How longitudinal and relatedness information is derived

Community composition
Bacteroides 12.4%Faecalibacterium 9.2%Phocaeicola 8.1%Alistipes 6.0%Prevotella 6.0%Blautia_A 3.8%Roseburia 3.4%Vescimonas 1.8%Lachnospira 1.7%Parabacteroides 1.7%Acetatifactor 1.5%Gemmiger 1.4%not assigned to a genus 6.5%other genera 36.4%

Genera, mean relative abundance over 272 profiled gut samples of this project (Sandpiper SingleM profiles, GTDB R232; low-depth and non-metagenome profiles left out).

Field coverage
fieldsamplescoverageR1R2R3R4
age_category522 100%
country522 100%522
health_condition522 100%522
subject_id471 90%471
collection_date522 100%522
detailed_location522 100%
health_condition_detail522 100%522
latitude522 100%522
location_locality522 100%522
location_region522 100%522
location_site522 100%522
longitude522 100%522
sex522 100%522
timepoint_label285 55%285
Papers (2)
  • Individuality and ethnicity eclipse a short-term dietary intervention in shaping microbiomes and viromes. 2022 PMID 35998206 PMC9397868 own_data
  • Characterization of the phyllosphere virome of fresh vegetables and potential transfer to the human gut. 2025 PMID 40210629 PMC11986028 unsure
Details
Archive description

Oral and gut metagenomes from female subjects

How this project entered the registry

Classified as human gut / stool / oral cavity shotgun DNA metagenome of age unknown by sonnet_x2 with confidence 0.60. Assay shotgun_dna.

  • host run.host_tax_id · “9606”
  • host study.description · “Oral and gut metagenomes from female subjects”
  • body site run.scientific_name · “feces metagenome(220)”
  • body site run.scientific_name · “oral metagenome(71)”
Evidence examples (one per field × route)
samplefieldvaluerouteconf.sourcequote
SAMN19188900collection_date2018/2019R10.90biosample.attribute:collection_date2018/2019
SAMN19188900countryUSR10.90sample.attr.geo_loc_nameUSA
SAMN19188900health_conditionhealthy_controlR30.70paper.fulltext.methodsHealthy women (age 18 to 40 years
SAMN19188900health_condition_detailHealthy womenR30.70paper.fulltext.methodsHealthy women (age 18 to 40 years
SAMN19188900latitude36.1486R10.90biosample.attribute:lat_lon36.1486 N 86.8050 W
SAMN19188900location_localityNashvilleR30.70paper.fulltext.methodsrecruited from the greater area of Nashville, Tennessee, USA
SAMN19188900location_regionTennesseeR10.60biosample.attribute:lat_lon36.1486 N 86.8050 W
SAMN19188900location_siteVanderbilt Center for Human NutritionR30.70paper.fulltext.methodsvisit to the Vanderbilt Center for Human Nutrition
SAMN19188900longitude-86.8050R10.90biosample.attribute:lat_lon36.1486 N 86.8050 W
SAMN19188900sexfemaleR30.70paper.fulltext.methodsThirty-eight female adult participants
SAMN19188938subject_idmb01R10.85sample.attr.subjectmb01
SAMN19188938timepoint_labelBeforeR10.85biosample.attribute:stagestage: Before
Sequencing

522 runs over 522 samples · mean 3.02 Gbp per run (median 2.29 Gbp, total 1,574.17 Gbp; 522 runs with a base count) · layout PAIRED 522 · platform ILLUMINA 522 · models Illumina NovaSeq 6000 (522) · 61% of runs with a Sandpiper profile · first public 2021-05-18 – 2022-09-30

Authors (25)

Li J first · George Markowitz RH · Brooks AW · Mallott EK · Leigh BA · Olszewski T · Zare H · Bagheri M · Smith HM · Friese KA · Habibi I · Lawrence WM · Rost CL · Lédeczi · Eeds AM · Ferguson JF · Silver HJ · Bordenstein SR last · Park JW first · Yun YE · Cho JA · Yoon SI · In SA · Park EJ · Kim MS last

Downloads

PRJNA662107.csv.gz (13.8 KB) · parquet (89.7 KB) — all 522 samples, every field with route and confidence · PRJNA662107_determinations.csv.gz (30.9 KB) — 5,976 evidence rows

Samples (522 · every field this project has values for · hover a cell for its source)
Loading the sample table…