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PRJNA665854 SRP285652

Gut & Oral Microbiome in Pancreatic Cancer, Kanazawa, Japan

WGS of feces and saliva of pancreatic cancer patients

57 fecal/saliva/tissue samples from pancreatic cancer patients and healthy controls in Kanazawa, Japan, analyzed by whole-genome sequencing in a case-control design. machine-written summary

57 samples · 57 runs · JP · Adult · 2020 · 0.51 Gbp / sample

Sources
✓Sequence archive 456○Abstract / description○Full text○Supplementary tables–External resources–User contribution–Human verified

Checked this project's papers and archive records yourself? Mark it human-verified.

what each source gave
✓ Sequence archive456 sample values (collection date, country, health condition, health condition detail, latitude, location locality, longitude, subject id)
○ Abstract / descriptionread; no usable statement
○ Full textread; no usable statement or table · PMC11487005, PMC8263681
○ Supplementary tablesfiles read; no table could be matched to the samples
– External resourcesnot covered by the external resources we ingest
– User contributionnothing uploaded yet
– Human verifiednot yet checked by a person · I checked this project — verify it
Samples at a glance
1 sampling locations · 1 country
Age
adult57
Condition
other_cancer39
healthy_control18
Country
JP57
Body site
primary42
excluded15
Collected 2014–2019
2014: 12 samples2015: 13 samples2018: 14 samples2019: 18 samples20142019
Sequencing depth per sample · this project all projects
all projects, 0–0.03 Gbp: 25,080 samples (4 %)all projects, 0.03–0.1 Gbp: 17,066 samples (3 %)all projects, 0.1–0.3 Gbp: 29,179 samples (5 %)this project, 0.1–0.3 Gbp: 4 samplesall projects, 0.3–1 Gbp: 47,044 samples (8 %)this project, 0.3–1 Gbp: 49 samplesall projects, 1–3 Gbp: 124,983 samples (22 %)this project, 1–3 Gbp: 4 samplesall projects, 3–10 Gbp: 253,728 samples (44 %)all projects, 10–30 Gbp: 72,533 samples (13 %)all projects, 30–100 Gbp: 3,024 samples (1 %)all projects, 100–300 Gbp: 106 samples (0 %)all projects, ≥ 300 Gbp: 9 samples (0 %)project median 0.51 Gbp per sample0130Gbp
Community composition

No Sandpiper profile for this project's samples yet.

Field coverage
fieldsamplescoverageR1R2R3R4
age_category57 100%
country57 100%57
health_condition57 100%57
subject_id57 100%57
collection_date57 100%57
detailed_location57 100%
health_condition_detail57 100%57
latitude57 100%57
location_locality57 100%57
longitude57 100%57
Papers (2)
  • Dysbiotic gut microbiota in pancreatic cancer patients form correlation networks with the oral microbiota and prognostic factors. 2021 PMID 34249452 PMC8263681 own_data
  • The microbial composition of pancreatic ductal adenocarcinoma: a systematic review of 16S rRNA gene sequencing. 2024 PMID 38874485 PMC11487005 unsure
Details
Archive description

Fecal and salivary DNA collected from pancreatic cancer patients or healthy controls were sequenced by WGS

How this project entered the registry

Classified as human gut / stool / oral cavity shotgun DNA metagenome of adult by deterministic_prior with confidence 0.90. Assay shotgun_dna.

  • host external_curation.infant_catalog · “infant triage reason_code age_adult_only”
  • body site study.title · “WGS of feces and saliva of”
  • body site study.description · “Fecal and salivary DNA”
  • body site study.title · “of feces and saliva of pancreatic cancer”
  • body site study.description · “Fecal and salivary DNA collected from”
  • life stage external_curation.infant_catalog · “infant triage reason_code age_adult_only”
Evidence examples (one per field × route)
samplefieldvaluerouteconf.sourcequote
SAMN16273809collection_date2014-06-30R10.90biosample.attribute:collection_date2014-06-30
SAMN16273809countryJPR10.90sample.attr.geo_loc_nameJapan: Kanazawa
SAMN16273809health_conditionhealthy_controlR10.70sample.name_rule:source_material_idHD07
SAMN16273809health_condition_detailHDR10.70sample.name_rule:source_material_idHD07
SAMN16273809latitude36.5500R10.90biosample.attribute:lat_lon36.55 N 136.67 E
SAMN16273809location_localityKanazawaR10.85biosample.attribute:geo_loc_nameJapan: Kanazawa
SAMN16273809longitude136.6700R10.90biosample.attribute:lat_lon36.55 N 136.67 E
SAMN16273809subject_idHD07R10.70sample.name_rule:source_material_idHD07
Sequencing

57 runs over 57 samples · mean 0.53 Gbp per run (median 0.51 Gbp, total 30.32 Gbp; 57 runs with a base count) · layout PAIRED 52, SINGLE 5 · platform ILLUMINA 57 · models Illumina MiSeq (57) · 5% of runs with a Sandpiper profile · first public 2020-09-30 – 2020-10-01

Authors (49)

Matsukawa H first · Iida N · Kitamura K · Terashima T · Seishima J · Makino I · Kannon T · Hosomichi K · Yamashita T · Sakai Y · Honda M · Mizukoshi E · Kaneko S last · Argentini C first · Lugli GA · Tarracchini C · Fontana F · Mancabelli L · Viappiani A · Anzalone R · Angelini L · Alessandri G · Longhi G · Bianchi MG · Taurino G · Bussolati O · Milani C · van Sinderen D · Turroni F · Ventura M last · Merali N first · Chouari T · Sweeney C · Halle-Smith J · Jessel MD · Wang B · O' Brien J · Suyama S · Jiménez JI · Roberts KJ · Velliou E · Sivakumar S · Rockall TA · Demirkan A · Pedicord V · Deng D · Giovannetti E · Annels NE · Frampton AE last

Downloads

PRJNA665854.csv.gz (2.5 KB) · parquet (72.1 KB) — all 57 samples, every field with route and confidence · PRJNA665854_determinations.csv.gz (4.1 KB) — 456 evidence rows

Samples (57 · every field this project has values for · hover a cell for its source)
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