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PRJNA725020 SRP325692

CBA/J mouse gut metagenome, Salmonella infection, Ohio

Fecal Metagenomes

58 fecal metagenome samples from CBA/J mice in Ohio, USA, comparing untreated animals to those infected with Salmonella to assess gut microbiome and viral genome changes during inflammation. machine-written summary

58 samples · 58 runs · US · Age unknown · 2021 · 14.71 Gbp / sample

Sources
✓Sequence archive 348○Abstract / description✓Full text 58✓Supplementary tables 216–External resources–User contribution–Human verified

Checked this project's papers and archive records yourself? Mark it human-verified.

what each source gave
✓ Sequence archive348 sample values (collection date, country, latitude, location region, longitude, subject id)
○ Abstract / descriptionread; no usable statement
✓ Full text58 sample values (location locality) · PMC10199544, PMC10653893
✓ Supplementary tables216 sample values (age, bmi, sex, smoking status)
– External resourcesnot covered by the external resources we ingest
– User contributionnothing uploaded yet
– Human verifiednot yet checked by a person · I checked this project — verify it
Samples at a glance
2 sampling locations · 1 country
Age
adult52
unknown4
elderly2
Sex
male31
female23
Country
US58
Body site
primary58
Collected 2017–2018
2017: 44 samples2018: 14 samples20172018
Sequencing depth per sample · this project all projects
all projects, 0–0.03 Gbp: 25,080 samples (4 %)all projects, 0.03–0.1 Gbp: 17,066 samples (3 %)all projects, 0.1–0.3 Gbp: 29,179 samples (5 %)all projects, 0.3–1 Gbp: 47,044 samples (8 %)all projects, 1–3 Gbp: 124,983 samples (22 %)all projects, 3–10 Gbp: 253,728 samples (44 %)this project, 3–10 Gbp: 4 samplesall projects, 10–30 Gbp: 72,533 samples (13 %)this project, 10–30 Gbp: 49 samplesall projects, 30–100 Gbp: 3,024 samples (1 %)this project, 30–100 Gbp: 5 samplesall projects, 100–300 Gbp: 106 samples (0 %)all projects, ≥ 300 Gbp: 9 samples (0 %)project median 14.71 Gbp per sample0130Gbp
Community composition
Bacteroides 17.7%Phocaeicola 11.8%Prevotella 9.7%Alistipes 6.5%Faecalibacterium 3.3%Roseburia 2.9%Escherichia 2.4%Parabacteroides 2.2%Succinivibrio 2.0%Lachnospira 1.6%Blautia_A 1.2%Sutterella 1.2%not assigned to a genus 3.4%other genera 34.1%

Genera, mean relative abundance over 54 profiled gut samples of this project (Sandpiper SingleM profiles, GTDB R232; low-depth and non-metagenome profiles left out).

Field coverage
fieldsamplescoverageR1R2R3R4
age_category54 93%
country58 100%58
health_condition0 0%
subject_id58 100%58
collection_date58 100%58
detailed_location58 100%
latitude58 100%58
location_locality58 100%58
location_region58 100%58
longitude58 100%58
age_at_collection_days54 93%54
bmi54 93%54
sex54 93%54
smoking_status54 93%54
Papers (2)
  • Exposing new taxonomic variation with inflammation - a murine model-specific genome database for gut microbiome researchers. 2023 PMID 37210515 PMC10199544 own_data
  • Targeted curation of the gut microbial gene content modulating human cardiovascular disease. 2023 PMID 37695138 PMC10653893 own_data
Details
Archive description

Fecal Metagenomes

How this project entered the registry

Classified as human gut / stool shotgun DNA metagenome of age unknown by sonnet_x2 with confidence 0.70. Assay shotgun_dna.

  • host run.host_tax_id · “9606”
  • body site run.scientific_name · “human gut metagenome”
Evidence examples (one per field × route)
samplefieldvaluerouteconf.sourcequote
SAMN18867751age_at_collection_days15706R20.85paper.supp.mbio.01511-23-s0001.xlsx[Tab 3!Age]43
SAMN18867751bmi41.8R20.85paper.supp.mbio.01511-23-s0001.xlsx[Tab 3!BMI]41.76774691
SAMN18867751collection_date2017R10.90biosample.attribute:collection_date2017
SAMN18867751countryUSR10.90sample.attr.geo_loc_nameUSA:Ohio
SAMN18867751latitude39.0000R10.90biosample.attribute:lat_lon39 N 84 W
SAMN18867751location_localityCincinnatiR30.70paper.fulltext.methodsUniversity of Cincinnati College of Medicine
SAMN18867751location_regionOhioR10.85biosample.attribute:geo_loc_nameUSA:Ohio
SAMN18867751longitude-84.0000R10.90biosample.attribute:lat_lon39 N 84 W
SAMN18867751sexmaleR20.85paper.supp.mbio.01511-23-s0001.xlsx[Tab 3!Sex]M
SAMN18867751smoking_statusneverR20.85paper.supp.mbio.01511-23-s0001.xlsx[Tab 3!Smoker]N
SAMN18867751subject_idP1R10.85biosample.attribute:aliasalias: P1
Sequencing

58 runs over 58 samples · mean 17.62 Gbp per run (median 14.71 Gbp, total 1,021.79 Gbp; 58 runs with a base count) · layout PAIRED 58 · platform ILLUMINA 58 · models Illumina NovaSeq 6000 (58) · 93% of runs with a Sandpiper profile · first public 2021-06-27 – 2021-06-28

Authors (22)

Leleiwi I first · Rodriguez-Ramos J · Shaffer M · Sabag-Daigle A · Kokkinias K · Flynn RM · Daly RA · Kop LFM · Solden LM · Ahmer BMM · Borton MA · Wrighton KC last · Hoyt DW · Jiang R · Ellenbogen JB · Purvine S · Nicora CD · Eder EK · Wong AR · Smulian AG · Lipton MS · Krzycki JA

Downloads

PRJNA725020.csv.gz (3.0 KB) · parquet (73.3 KB) — all 58 samples, every field with route and confidence · PRJNA725020_determinations.csv.gz (5.8 KB) — 622 evidence rows

Samples (58 · every field this project has values for · hover a cell for its source)
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