Gut microbiota in ALS patients vs caregivers, Modena, Italy
Case-control study of 22 fecal samples from ALS patients and healthy family caregivers in Modena, Italy, comparing gut microbiota (16S) and inflammatory cytokine profiles across clinical phenotypes. machine-written summary
22 samples · 22 runs · IT · Adult · 2021 · 75.9 Mbp / sample
Checked this project's papers and archive records yourself? Mark it human-verified.
what each source gave
| ✓ Sequence archive | 253 sample values (age, collection date, country, health condition, health condition detail, latitude, location locality, location region) |
| ○ Abstract / description | read; no usable statement |
| ✓ Full text | 44 sample values (antibiotic exposure, location site) · PMC8301418 |
| ○ Supplementary tables | files read; no table could be matched to the samples |
| – External resources | not covered by the external resources we ingest |
| – User contribution | nothing uploaded yet |
| – Human verified | not yet checked by a person · I checked this project — verify it |
families — not resolved
19 ALS patients vs 9 healthy family caregivers (controls), matched for sex and age; no individual dyad IDs given.
No Sandpiper profile for this project's samples yet.
| field | samples | coverage | R1 | R2 | R3 | R4 |
|---|---|---|---|---|---|---|
| age_category | 22 | 100% | ||||
| country | 22 | 100% | 22 | |||
| health_condition | 22 | 100% | 22 | |||
| subject_id | 22 | 100% | 22 | |||
| age_at_collection_days | 22 | 100% | 22 | |||
| antibiotic_exposure | 22 | 100% | 22 | |||
| collection_date | 22 | 100% | 22 | |||
| detailed_location | 22 | 100% | ||||
| health_condition_detail | 22 | 100% | 22 | |||
| latitude | 22 | 100% | 22 | |||
| location_locality | 22 | 100% | 22 | |||
| location_region | 22 | 100% | 22 | |||
| location_site | 22 | 100% | 22 | |||
| longitude | 22 | 100% | 22 | |||
| sex | 22 | 100% | 22 | |||
| timepoint_label | 11 | 50% | 11 |
- The Gut Microbiota-Immunity Axis in ALS: A Role in Deciphering Disease Heterogeneity? 2021 PMID 34209688 PMC8301418 own_data
Archive description
Amyotrophic Lateral Sclerosis (ALS) is a neurodegenerative disorder with unknown etiology and no effective treatment, characterized by large phenotypic variabilityheterogeneity, with respect to thevariable site and age of symptom onset and rate of disease progression. Considerable studies support the role of the microbiota-immunity axis in the pathogenesis of neurodegenerative dis-eases. In the present study, we compared the inflammatory and microbiota profile of ALS patients with different clinical characteristics with healthy family caregivers.
How this project entered the registry
Classified as human gut / stool shotgun DNA metagenome of adult and elderly by sonnet_x2 with confidence 0.55. Assay shotgun_dna.
- host run.host_tax_id · “9606”
- host run.scientific_name · “human gut metagenome”
- body site study.title · “gut microbiota of Amyotrophic Lateral Sclerosis”
- life stage study.description · “variable site and age of symptom onset”
- life stage sample.attr.host_age · “65 (n=8/22)”
Evidence examples (one per field × route)
| sample | field | value | route | conf. | source | quote |
|---|---|---|---|---|---|---|
| SAMN19897755 | age_at_collection_days | 21549.8 | R1 | 0.70 | sample.attr.host_age | 59 |
| SAMN19897755 | antibiotic_exposure | no | R3 | 0.70 | paper.fulltext.methods | antimicrobial treatment four weeks prior to sample collection |
| SAMN19897755 | collection_date | 2017 | R1 | 0.90 | biosample.attribute:collection_date | 2017 |
| SAMN19897755 | country | IT | R1 | 0.90 | sample.attr.geo_loc_name | Italy: Modena |
| SAMN19897755 | health_condition | healthy_control | R1 | 0.70 | biosample.name_rule:DESCRIPTION | CSLA1 |
| SAMN19897762 | health_condition | neurological_psychiatric | R1 | 0.90 | biosample_attr | ALS |
| SAMN19897755 | health_condition_detail | healthy family caregiver (CSLA control) | R1 | 0.70 | biosample.name_rule:DESCRIPTION | CSLA1 |
| SAMN19897762 | health_condition_detail | ALS | R1 | 0.90 | sample.attr.host_disease | ALS |
| SAMN19897755 | latitude | 44.6345 | R1 | 0.90 | biosample.attribute:lat_lon | 44.63447765 N 10.94067400 E |
| SAMN19897755 | location_locality | Modena | R1 | 0.85 | biosample.attribute:geo_loc_name | Italy: Modena |
| SAMN19897755 | location_region | Emilia-Romagna | R1 | 0.60 | biosample.attribute:lat_lon | 44.63447765 N 10.94067400 E |
| SAMN19897755 | location_site | tertiary ALS Center in Modena | R3 | 0.70 | paper.fulltext.methods | recruited by the tertiary ALS Center in Modena |
| SAMN19897755 | longitude | 10.9407 | R1 | 0.90 | biosample.attribute:lat_lon | 44.63447765 N 10.94067400 E |
| SAMN19897755 | sex | female | R1 | 0.90 | sample.attr.host_sex | female |
| SAMN19897755 | subject_id | CSLA1 | R1 | 0.70 | sample.name_rule:TITLE | CSLA1 |
| SAMN19897763 | timepoint_label | T0 | R1 | 0.70 | biosample.name_rule:DESCRIPTION | SLA1_T0 |
Sequencing
22 runs over 22 samples · mean 0.13 Gbp per run (median 75.9 Mbp, total 2.85 Gbp; 22 runs with a base count) · layout PAIRED 22 · platform ILLUMINA 22 · models Illumina MiSeq (22) · 0% of runs with a Sandpiper profile · first public 2021-07-04
Authors (11)
Niccolai E first · Di Pilato V · Nannini G · Baldi S · Russo E · Zucchi E · Martinelli I · Menicatti M · Bartolucci G · Mandrioli J · Amedei A last
Downloads
PRJNA736213.csv.gz (1.8 KB) · parquet (72.4 KB) — all 22 samples, every field with route and confidence · PRJNA736213_determinations.csv.gz (3.5 KB) — 297 evidence rows