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PRJNA739858 SRP325679

Gut metagenome of healthy elderly adults, Singapore

Gut microbiome analysis of the Brain, Adipocyte, Microbiome, Muscle, Bone, Exercise (BAMMBE) elderly Singapore Cohort

Cross-sectional metagenomic study of 121 gut microbiome samples from healthy community-dwelling elderly participants in Singapore's BAMMBE cohort, examining lifestyle factors in relation to gut microbiome, health status, and cognitive functioning. machine-written summary

121 samples · 121 runs · SG · Elderly · 2022 · 1.42 Gbp / sample

Sources
✓Sequence archive 605✓Abstract / description 121–Full text–Supplementary tables–External resources–User contribution–Human verified

Checked this project's papers and archive records yourself? Mark it human-verified.

what each source gave
✓ Sequence archive605 sample values (collection date, country, latitude, location locality, longitude)
✓ Abstract / description121 sample values (health condition)
– Full textno open-access full text linked
– Supplementary tablesno supplementary tables available (or none open access)
– External resourcesnot covered by the external resources we ingest
– User contributionnothing uploaded yet
– Human verifiednot yet checked by a person · I checked this project — verify it
Samples at a glance
1 sampling locations · 1 country
Age
elderly121
Condition
healthy_control121
Country
SG121
Body site
primary121
Sequencing depth per sample · this project all projects
all projects, 0–0.03 Gbp: 25,080 samples (4 %)all projects, 0.03–0.1 Gbp: 17,066 samples (3 %)all projects, 0.1–0.3 Gbp: 29,179 samples (5 %)all projects, 0.3–1 Gbp: 47,044 samples (8 %)all projects, 1–3 Gbp: 124,983 samples (22 %)this project, 1–3 Gbp: 121 samplesall projects, 3–10 Gbp: 253,728 samples (44 %)all projects, 10–30 Gbp: 72,533 samples (13 %)all projects, 30–100 Gbp: 3,024 samples (1 %)all projects, 100–300 Gbp: 106 samples (0 %)all projects, ≥ 300 Gbp: 9 samples (0 %)project median 1.42 Gbp per sample0130Gbp
Community composition
Bacteroides 19.3%Phocaeicola 14.1%Prevotella 11.5%Alistipes 4.9%Roseburia 3.3%Faecalibacterium 3.1%Parabacteroides 2.6%Lachnospira 1.5%Blautia_A 1.5%Megamonas 1.3%Acetatifactor 1.1%Sutterella 1.1%not assigned to a genus 7.1%other genera 27.5%

Genera, mean relative abundance over 108 profiled gut samples of this project (Sandpiper SingleM profiles, GTDB R232; low-depth and non-metagenome profiles left out).

Field coverage
fieldsamplescoverageR1R2R3R4
age_category121 100%
country121 100%121
health_condition121 100%121
subject_id0 0%
collection_date121 100%121
detailed_location121 100%
latitude121 100%121
location_locality121 100%121
longitude121 100%121
Details
Archive description

Here, we report metagenomic sequencing (2x250 bp paired-end reads) of gut microbial profiles from healthy community-dwelling elderly participants from the Brain, Adipocyte, Microbiome, Muscle, Bone, Exercise (BAMMBE) observational cohort, which was designed to understand the role of lifestyle factors on the gut microbiome, health status and cognitive functioning.

How this project entered the registry

Classified as human gut / stool shotgun DNA metagenome of elderly by sonnet_x2 with confidence 0.75. Assay shotgun_dna.

  • host run.host_tax_id · “9606”
  • body site study.title · “Gut microbiome analysis”
  • life stage study.title · “BAMMBE elderly Singapore Cohort”
Evidence examples (one per field × route)
samplefieldvaluerouteconf.sourcequote
SAMN19803454collection_date2019-02-20R10.90biosample.attribute:collection_date2019-02-20
SAMN19803454countrySGR10.90sample.attr.geo_loc_nameSingapore
SAMN19803454health_conditionhealthy_controlR40.50study.descriptionhealthy community-dwelling elderly participants
SAMN19803454latitude1.3521R10.90biosample.attribute:lat_lon1.3521 N 103.8198 E
SAMN19803454location_localitySingaporeR10.85biosample.attribute:geo_loc_nameSingapore
SAMN19803454longitude103.8198R10.90biosample.attribute:lat_lon1.3521 N 103.8198 E
Sequencing

121 runs over 121 samples · mean 1.43 Gbp per run (median 1.42 Gbp, total 172.86 Gbp; 121 runs with a base count) · layout PAIRED 121 · platform ILLUMINA 121 · models Illumina HiSeq 2500 (121) · 89% of runs with a Sandpiper profile · first public 2022-05-31

Authors (0)

No author record.

Downloads

PRJNA739858.csv.gz (3.8 KB) · parquet (74.6 KB) — all 121 samples, every field with route and confidence · PRJNA739858_determinations.csv.gz (4.9 KB) — 726 evidence rows

Samples (121 · every field this project has values for · hover a cell for its source)
Loading the sample table…