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PRJNA740337 SRP325397

Cultivation of skin, oral, and gut bacteria, Farmington USA

Cultivation of dominant bacterial species and strains from human skin, oral, and gut microbiota

46 samples from Farmington, USA, used to cultivate dominant bacterial species and strains from skin, oral, and gut microbiota via a metagenomics- and mass spectrometry-informed cultivation pipeline. machine-written summary

46 samples · 46 runs · US · Age unknown · 2022 · 0.42 Gbp / sample

Sources
✓Sequence archive 322○Abstract / description○Full text○Supplementary tables–External resources–User contribution–Human verified

Checked this project's papers and archive records yourself? Mark it human-verified.

what each source gave
✓ Sequence archive322 sample values (collection date, country, latitude, location locality, location region, longitude, subject id)
○ Abstract / descriptionread; no usable statement
○ Full textread; no usable statement or table · PMC8515726
○ Supplementary tablesfiles read; no table could be matched to the samples
– External resourcesnot covered by the external resources we ingest
– User contributionnothing uploaded yet
– Human verifiednot yet checked by a person · I checked this project — verify it
Samples at a glance
1 sampling locations · 1 country
Age
unknown46
Country
US46
Body site
excluded26
primary20
Sequencing depth per sample · this project all projects
all projects, 0–0.03 Gbp: 25,080 samples (4 %)all projects, 0.03–0.1 Gbp: 17,066 samples (3 %)this project, 0.03–0.1 Gbp: 1 samplesall projects, 0.1–0.3 Gbp: 29,179 samples (5 %)this project, 0.1–0.3 Gbp: 15 samplesall projects, 0.3–1 Gbp: 47,044 samples (8 %)this project, 0.3–1 Gbp: 25 samplesall projects, 1–3 Gbp: 124,983 samples (22 %)this project, 1–3 Gbp: 4 samplesall projects, 3–10 Gbp: 253,728 samples (44 %)this project, 3–10 Gbp: 1 samplesall projects, 10–30 Gbp: 72,533 samples (13 %)all projects, 30–100 Gbp: 3,024 samples (1 %)all projects, 100–300 Gbp: 106 samples (0 %)all projects, ≥ 300 Gbp: 9 samples (0 %)project median 0.42 Gbp per sample0130Gbp
Sampling design and relatedness

longitudinal — not resolved

4 of 6 people sampled more than once (18 samples; median 4, up to 5 per person).

How longitudinal and relatedness information is derived

Community composition
Bacteroides 31.8%Phocaeicola 17.9%Prevotella 7.0%Zag111 5.4%Alistipes 4.0%Roseburia 2.7%Parabacteroides 2.6%Faecalibacterium 2.4%Phascolarctobacterium 2.1%Paraprevotella 1.6%Enterocloster 1.1%Otoolea 1.1%not assigned to a genus 6.3%other genera 14.0%

Genera, mean relative abundance over 4 profiled gut samples of this project (Sandpiper SingleM profiles, GTDB R232; low-depth and non-metagenome profiles left out).

Field coverage
fieldsamplescoverageR1R2R3R4
age_category0 0%
country46 100%46
health_condition0 0%
subject_id46 100%46
collection_date46 100%46
detailed_location46 100%
latitude46 100%46
location_locality46 100%46
location_region46 100%46
longitude46 100%46
Papers (1)
  • Cultivation of common bacterial species and strains from human skin, oral, and gut microbiota. 2021 PMID 34649516 PMC8515726 own_data
Details
Archive description

Cultivation of dominant bacterial species and strains from human skin, oral, and gut microbiota

How this project entered the registry

Classified as human skin / oral cavity / gut / stool / other human site shotgun DNA metagenome of age unknown by sonnet_x2 with confidence 0.55. Assay shotgun_dna.

  • host run.host_tax_id · “9606”
  • host run.scientific_name · “human oral metagenome”
  • body site study.title · “human skin, oral, and gut microbiota”
  • body site run.scientific_name · “human skin metagenome”
  • body site sample.attr.isolation_source · “toeweb (n=5/46)”
Evidence examples (one per field × route)
samplefieldvaluerouteconf.sourcequote
SAMN19840714collection_date2019R10.90biosample.attribute:collection_date2019
SAMN19840714countryUSR10.90sample.attr.geo_loc_nameUSA: Farmington
SAMN19840714latitude41.7334R10.90biosample.attribute:lat_lon41.7334221 N 72.794137 W
SAMN19840714location_localityFarmingtonR10.85biosample.attribute:geo_loc_nameUSA: Farmington
SAMN19840714location_regionConnecticutR10.60biosample.attribute:lat_lon41.7334221 N 72.794137 W
SAMN19840714longitude-72.7941R10.90biosample.attribute:lat_lon41.7334221 N 72.794137 W
SAMN19840714subject_id1000R10.70sample.name_rule:sample_alias1000.MSR-1
Sequencing

46 runs over 46 samples · mean 0.73 Gbp per run (median 0.42 Gbp, total 33.46 Gbp; 46 runs with a base count) · layout PAIRED 46 · platform ILLUMINA 46 · models Illumina HiSeq 2500 (46) · 11% of runs with a Sandpiper profile · first public 2022-04-29

Authors (15)

Fleming E first · Pabst V · Scholar Z · Xiong R · Voigt AY · Zhou W · Hoyt A · Hardy R · Peterson A · Beach R · Ondouah-Nzutchi Y · Dong J · Bateman L · Vernon SD · Oh J last

Downloads

PRJNA740337.csv.gz (2.0 KB) · parquet (71.0 KB) — all 46 samples, every field with route and confidence · PRJNA740337_determinations.csv.gz (2.8 KB) — 322 evidence rows

Samples (46 · every field this project has values for · hover a cell for its source)
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