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PRJNA755720 SRP333105

Western vs Mediterranean diet effects on gut microbiota

Differential effects of western and mediterranean-type diets on gut microbiota: a metagenomics and metabolomics approach

35 shotgun metagenome samples from adults in Barcelona, Spain, studying effects of western and Mediterranean-type diets on gut microbiota as part of a dietary intervention design. machine-written summary

35 samples · 35 runs · ES · Adult · 2021 · 3.67 Gbp / sample

Sources
✓Sequence archive 315✓Abstract / description 70○Full text○Supplementary tables–External resources–User contribution–Human verified

Checked this project's papers and archive records yourself? Mark it human-verified.

what each source gave
✓ Sequence archive315 sample values (collection date, country, diet, diet detail, intervention, intervention detail, location locality, sex)
✓ Abstract / description70 sample values (health condition, health condition detail)
○ Full textread; no usable statement or table · PMC8400818
○ Supplementary tablesfiles read; no table could be matched to the samples
– External resourcesnot covered by the external resources we ingest
– User contributionnothing uploaded yet
– Human verifiednot yet checked by a person · I checked this project — verify it
Samples at a glance
1 country
Age
adult35
Condition
healthy_control35
Sex
male35
Country
ES35
Body site
primary35
Sequencing depth per sample · this project all projects
all projects, 0–0.03 Gbp: 25,080 samples (4 %)all projects, 0.03–0.1 Gbp: 17,066 samples (3 %)all projects, 0.1–0.3 Gbp: 29,179 samples (5 %)all projects, 0.3–1 Gbp: 47,044 samples (8 %)this project, 0.3–1 Gbp: 1 samplesall projects, 1–3 Gbp: 124,983 samples (22 %)this project, 1–3 Gbp: 9 samplesall projects, 3–10 Gbp: 253,728 samples (44 %)this project, 3–10 Gbp: 25 samplesall projects, 10–30 Gbp: 72,533 samples (13 %)all projects, 30–100 Gbp: 3,024 samples (1 %)all projects, 100–300 Gbp: 106 samples (0 %)all projects, ≥ 300 Gbp: 9 samples (0 %)project median 3.67 Gbp per sample0130Gbp
Sampling design and relatedness

longitudinal — not resolved

15 of 20 people sampled more than once (30 samples; median 2, up to 2 per person). Schedule stated by the source: “Cross-over: 2-week washout diet, then 2-week WD or FMD diet period, then crossover; faecal samples from last days of each diet period” (time origin: intervention_start).

How longitudinal and relatedness information is derived

Community composition
Prevotella 14.8%Bacteroides 7.7%Phocaeicola 6.4%Roseburia 4.3%Faecalibacterium 4.0%Alistipes 3.7%Ruminococcoides 2.6%Vescimonas 2.0%Hominicoprocola 2.0%Bifidobacterium 1.9%Dialister 1.8%Blautia_A 1.7%not assigned to a genus 7.1%other genera 40.0%

Genera, mean relative abundance over 31 profiled gut samples of this project (Sandpiper SingleM profiles, GTDB R232; low-depth and non-metagenome profiles left out).

Field coverage
fieldsamplescoverageR1R2R3R4
age_category35 100%
country35 100%35
health_condition35 100%35
subject_id35 100%35
collection_date35 100%35
detailed_location35 100%
diet35 100%35
diet_detail35 100%35
health_condition_detail35 100%35
intervention35 100%35
intervention_detail35 100%35
location_locality35 100%35
sex35 100%35
Papers (1)
  • Differential Effects of Western and Mediterranean-Type Diets on Gut Microbiota: A Metagenomics and Metabolomics Approach. 2021 PMID 34444797 PMC8400818 own_data
Details
Archive description

Shotgun metagenomics sequencing data for the article "Differential effects of western and mediterranean-type diets on gut microbiota: a metagenomics and metabolomics approach" (manuscript ID nutrients-1289360)

How this project entered the registry

Classified as human gut / stool shotgun DNA metagenome of adult by deterministic_prior with confidence 0.90. Assay shotgun_dna.

  • host external_curation.infant_catalog · “infant triage reason_code age_adult_only”
  • body site study.title · “mediterranean-type diets on gut microbiota: a metagenomics”
  • body site study.description · “mediterranean-type diets on gut microbiota: a metagenomics”
  • life stage external_curation.infant_catalog · “infant triage reason_code age_adult_only”
Evidence examples (one per field × route)
samplefieldvaluerouteconf.sourcequote
SAMN20837588collection_date2020-01-28R10.90biosample.attribute:collection_date2020-01-28
SAMN20837588countryESR10.90sample.attr.geo_loc_nameSpain: Barcelona
SAMN20837588diettherapeutic_or_study_dietR10.90biosample.attribute:host_dietFiber-enriched Mediterranean-type Diet (FMD)
SAMN20837588diet_detailFiber-enriched Mediterranean-type Diet (FMD)R10.90biosample.attribute:host_dietFiber-enriched Mediterranean-type Diet (FMD)
SAMN20837588health_conditionhealthy_controlR40.50paper.abstractIn 20 healthy men, each diet was administered for 2 weeks preceded by a 2-week washout diet
SAMN20837588health_condition_detailhealthy menR40.50paper.abstractIn 20 healthy men, each diet was administered for 2 weeks preceded by a 2-week washout diet
SAMN20837588interventiondiet_interventionR10.90biosample.attribute:host_diethost_diet: Fiber-enriched Mediterranean-type Diet (FMD)
SAMN20837588intervention_detailfiber-enriched mediterranean-type diet (FMD) armR10.90biosample.attribute:host_diethost_diet: Fiber-enriched Mediterranean-type Diet (FMD)
SAMN20837588location_localityBarcelonaR10.85biosample.attribute:geo_loc_nameSpain: Barcelona
SAMN20837588sexmaleR10.90sample.attr.host_sexmale
SAMN20837588subject_id1R10.70sample.name_rule:aliasED1F
Sequencing

35 runs over 35 samples · mean 3.52 Gbp per run (median 3.67 Gbp, total 123.37 Gbp; 35 runs with a base count) · layout PAIRED 35 · platform ILLUMINA 35 · models NextSeq 500 (35) · 89% of runs with a Sandpiper profile · first public 2021-08-25

Authors (0)

No author record.

Downloads

PRJNA755720.csv.gz (2.0 KB) · parquet (71.9 KB) — all 35 samples, every field with route and confidence · PRJNA755720_determinations.csv.gz (3.6 KB) — 385 evidence rows

Samples (35 · every field this project has values for · hover a cell for its source)
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