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PRJNA816918 SRP364577

Fecal Metagenomes from Healthy Adults in Davis, California

GENIUS Study, Shotgun Metagenomes from Fecal Samples of Healthy Adults

18 fecal metagenome samples from healthy adults with diverse diets in Davis, California, used in a cross-sectional study of fecal fermentations with sugar beet-derived oligosaccharides and related in vitro prebiotic/synbiotic experiments. machine-written summary

18 samples · 18 runs · US · Adult · 2025 · 11.77 Gbp / sample

Sources
✓Sequence archive 108✓Abstract / description 36✓Full text 18○Supplementary tables–External resources–User contribution–Human verified

Checked this project's papers and archive records yourself? Mark it human-verified.

what each source gave
✓ Sequence archive108 sample values (collection date, country, latitude, location locality, location region, longitude)
✓ Abstract / description36 sample values (health condition, health condition detail)
✓ Full text18 sample values (subject id) · PMC11921329, PMC12415832
○ Supplementary tablesfiles read; no table could be matched to the samples
– External resourcesnot covered by the external resources we ingest
– User contributionnothing uploaded yet
– Human verifiednot yet checked by a person · I checked this project — verify it
Samples at a glance
1 sampling locations · 1 country
Age
adult18
Condition
healthy_control18
Country
US18
Body site
primary18
Sequencing depth per sample · this project all projects
all projects, 0–0.03 Gbp: 25,080 samples (4 %)all projects, 0.03–0.1 Gbp: 17,066 samples (3 %)all projects, 0.1–0.3 Gbp: 29,179 samples (5 %)all projects, 0.3–1 Gbp: 47,044 samples (8 %)all projects, 1–3 Gbp: 124,983 samples (22 %)all projects, 3–10 Gbp: 253,728 samples (44 %)all projects, 10–30 Gbp: 72,533 samples (13 %)this project, 10–30 Gbp: 18 samplesall projects, 30–100 Gbp: 3,024 samples (1 %)all projects, 100–300 Gbp: 106 samples (0 %)all projects, ≥ 300 Gbp: 9 samples (0 %)project median 11.77 Gbp per sample0130Gbp
Community composition
Faecalibacterium 8.8%Prevotella 8.1%Blautia_A 5.9%Bacteroides 5.8%Phocaeicola 5.2%Roseburia 4.5%Bifidobacterium 4.3%Ruminococcoides 3.4%Gemmiger 3.0%Lachnospira 2.6%Alistipes 2.4%Fusicatenibacter 1.8%not assigned to a genus 3.3%other genera 41.0%

Genera, mean relative abundance over 17 profiled gut samples of this project (Sandpiper SingleM profiles, GTDB R232; low-depth and non-metagenome profiles left out).

Field coverage
fieldsamplescoverageR1R2R3R4
age_category18 100%
country18 100%18
health_condition18 100%18
subject_id18 100%18
collection_date18 100%18
detailed_location18 100%
health_condition_detail18 100%18
latitude18 100%18
location_locality18 100%18
location_region18 100%18
longitude18 100%18
Papers (2)
  • Generation of novel prebiotic oligosaccharide pools from fiber drives biological insight in bacterial glycan metabolism. 2025 PMID 39912642 PMC11921329 own_data
  • Fecal microbiomes from healthy adult consumers of fruits and vegetables exhibit fiber- and donor-specific fermentation: "5 a day" is not enough. 2025 PMID 40916941 PMC12415832 own_data
Details
Archive description

Shotgun metagenomes of fecal samples collected from healthy adults in California with diverse diets

How this project entered the registry

Classified as human gut / stool shotgun DNA metagenome of adult by sonnet_x2 with confidence 0.82. Assay shotgun_dna.

  • host run.host_tax_id · “9606”
  • body site study.description · “fecal samples collected from healthy adults”
  • life stage study.description · “fecal samples collected from healthy adults”
Evidence examples (one per field × route)
samplefieldvaluerouteconf.sourcequote
SAMN26733127collection_date2021-01-31R10.90biosample.attribute:collection_date2021-01-31T09:25:00Z
SAMN26733127countryUSR10.90sample.attr.geo_loc_nameUSA: Davis, California
SAMN26733127health_conditionhealthy_controlR40.40study.descriptionhealthy adults in California with diverse diets
SAMN26733127health_condition_detailAll subjects described as healthyR40.40study.descriptionhealthy adults in California with diverse diets
SAMN26733127latitude38.5449R10.90biosample.attribute:lat_lon38.5449 N 121.7405 W
SAMN26733127location_localityDavisR10.85biosample.attribute:geo_loc_nameUSA: Davis, California
SAMN26733127location_regionCaliforniaR10.85biosample.attribute:geo_loc_nameUSA: Davis, California
SAMN26733127longitude-121.7405R10.90biosample.attribute:lat_lon38.5449 N 121.7405 W
SAMN26733127subject_idSAMN26733127R30.75design.one_sample_per_subject:fulltextthus, a total of eighteen stool samples from eighteen participants were analyzed
Sequencing

18 runs over 18 samples · mean 12.14 Gbp per run (median 11.77 Gbp, total 218.54 Gbp; 18 runs with a base count) · layout PAIRED 18 · platform ILLUMINA 18 · models Illumina NovaSeq 6000 (18) · 94% of runs with a Sandpiper profile · first public 2025-05-04

Authors (25)

Masarweh C first · Maldonado-Gomez M · Paviani B · Bhattacharya M · Weng CY · Suarez C · Ehlers-Cheang S · Stacy A · Castillo J · Krishnakumar N · Kalanetra KA · Barile D · German JB · Lebrilla CB · Mills DA last · Blecksmith SE first · Kalanetra KM · Sitepu IR · Tang Y · Ehlers Cheang S · Jiang S · Cernioglo K · Damian-Medina K · Smilowitz JT · Lemay DG last

Downloads

PRJNA816918.csv.gz (1.5 KB) · parquet (71.0 KB) — all 18 samples, every field with route and confidence · PRJNA816918_determinations.csv.gz (2.0 KB) — 162 evidence rows

Samples (18 · every field this project has values for · hover a cell for its source)
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