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PRJNA832737 SRP372536

Gut microbiome, milk-intolerant vs milk drinkers, Italy

gut metagenome of subjects with GID upon milk consumption

25 gut metagenome samples from a case-control study of non-habitual milk consumers with gastro-intestinal discomfort (NHMC) versus habitual milk consumers without discomfort (HMC) following cow's milk consumption. machine-written summary

25 samples · 25 runs · Age unknown · 2022 · 8.96 Gbp / sample

Sources
✓Sequence archive 100○Abstract / description○Full text○Supplementary tables–External resources–User contribution–Human verified

Checked this project's papers and archive records yourself? Mark it human-verified.

what each source gave
✓ Sequence archive100 sample values (collection date, health condition, health condition detail, subject id)
○ Abstract / descriptionread; no usable statement
○ Full textread; no usable statement or table · PMC10948488
○ Supplementary tablesfiles read; no table could be matched to the samples
– External resourcesnot covered by the external resources we ingest
– User contributionnothing uploaded yet
– Human verifiednot yet checked by a person · I checked this project — verify it
Samples at a glance
Age
adult25
Condition
healthy_control13
other_disease12
Body site
primary25
Sequencing depth per sample · this project all projects
all projects, 0–0.03 Gbp: 25,080 samples (4 %)all projects, 0.03–0.1 Gbp: 17,066 samples (3 %)all projects, 0.1–0.3 Gbp: 29,179 samples (5 %)all projects, 0.3–1 Gbp: 47,044 samples (8 %)all projects, 1–3 Gbp: 124,983 samples (22 %)all projects, 3–10 Gbp: 253,728 samples (44 %)this project, 3–10 Gbp: 16 samplesall projects, 10–30 Gbp: 72,533 samples (13 %)this project, 10–30 Gbp: 9 samplesall projects, 30–100 Gbp: 3,024 samples (1 %)all projects, 100–300 Gbp: 106 samples (0 %)all projects, ≥ 300 Gbp: 9 samples (0 %)project median 8.96 Gbp per sample0130Gbp
Community composition
Blautia_A 10.5%Bifidobacterium 9.5%Roseburia 6.0%Faecalibacterium 5.5%Gemmiger 5.3%Collinsella 3.6%Ruminococcoides 3.1%Prevotella 2.6%Mediterraneibacter 2.5%Anaerostipes 2.4%Bacteroides 2.3%Phocaeicola 2.2%not assigned to a genus 3.6%other genera 40.9%

Genera, mean relative abundance over 24 profiled gut samples of this project (Sandpiper SingleM profiles, GTDB R232; low-depth and non-metagenome profiles left out).

Field coverage
fieldsamplescoverageR1R2R3R4
age_category25 100%
country0 0%
health_condition25 100%25
subject_id25 100%25
collection_date25 100%25
health_condition_detail25 100%25
Papers (1)
  • Relationships between diet and gut microbiome in an Italian and Dutch cohort: does the dietary protein to fiber ratio play a role? 2024 PMID 38151533 PMC10948488 own_data
Details
Archive description

gut metagenome of 19 non-habitual milk consumers with gastro-intestinal discomfort upon cow's milk consumption (NHMC) and 20 habitual milk consumers without GID (HMC), participating in a gut permeability and a milk load

How this project entered the registry

Classified as human gut / stool shotgun DNA metagenome of age unknown by sonnet_x2 with confidence 0.70. Assay shotgun_dna.

  • host run.scientific_name · “human gut metagenome”
  • host run.host_tax_id · “9606”
  • body site study.title · “gut metagenome of subjects with GID”
Evidence examples (one per field × route)
samplefieldvaluerouteconf.sourcequote
SAMN27917751collection_date2020R10.90biosample.attribute:collection_date2020
SAMN27917751health_conditionhealthy_controlR10.70sample.name_rule:attributeHMC subject D102
SAMN27917751health_condition_detailHMCR10.70sample.name_rule:attributeHMC subject D102
SAMN27917751subject_idD102R10.70sample.name_rule:attributeHMC subject D102
Sequencing

25 runs over 25 samples · mean 9.69 Gbp per run (median 8.96 Gbp, total 242.19 Gbp; 25 runs with a base count) · layout PAIRED 25 · platform ILLUMINA 25 · models Illumina NovaSeq 6000 (25) · 96% of runs with a Sandpiper profile · first public 2022-04-28

Authors (6)

Tagliamonte S first · Puhlmann ML · De Filippis F · Guerville M · Ercolini D · Vitaglione P last

Downloads

PRJNA832737.csv.gz (1.5 KB) · parquet (69.8 KB) — all 25 samples, every field with route and confidence · PRJNA832737_determinations.csv.gz (1.3 KB) — 100 evidence rows

Samples (25 · every field this project has values for · hover a cell for its source)
Loading the sample table…