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PRJNA844888 SRP378196

Microbiota transplant RCT in ulcerative colitis, Minneapolis

Vaughn UC IMT Study

188 gut microbiome samples from ulcerative colitis patients in Minneapolis, MN, USA, from a double-blind, placebo-controlled trial of encapsulated microbiota transplant therapy (MTT) versus placebo. machine-written summary

188 samples · 188 runs · US · Age unknown · 2025 · 7.79 Gbp / sample

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Sources

✓Sequence archive 1,417✓Abstract / description 376–Full text○Supplementary tables–External resources–User contribution–Human verified

Checked this project's papers and archive records yourself? Mark it human-verified.

what each source gave

✓ Sequence archive	1,417 sample values (country, intervention, latitude, location locality, location region, longitude, subject id, timepoint)
✓ Abstract / description	376 sample values (health condition, health condition detail)
– Full text	no open-access full text linked · PMC12476944
○ Supplementary tables	files read; no table could be matched to the samples
– External resources	not covered by the external resources we ingest
– User contribution	nothing uploaded yet
– Human verified	not yet checked by a person · I checked this project — verify it

Interventions

intervention — arms not resolved

Faecal microbiota transplantation design randomized_controlled_trial · population ulcerative_colitis · Encapsulated microbiota transplant therapy (MTP-101C) vs placebo capsules, 8 weeks, UC

“Ulcerative colitis patients received either encapsulated (drug name MTP-101C) or placebo capsules” — abstract:39240145 (study level; per-sample arms are in the intervention column below)

Samples at a glance

1 sampling locations · 1 country

Age

unknown188

Condition

ulcerative_colitis188

Country

US188

Body site

primary188

Sequencing depth per sample · this project all projects

Sampling design and relatedness

longitudinalFMT donor → recipient — not resolved

28 of 31 people sampled more than once (185 samples; median 6, up to 33 per person) · follow-up median 3 mo (max 3 mo) · median interval between samples 28 d (time from visit codes mapped to the stated schedule). Schedule stated by the source: “Daily capsules for 8 weeks, 4-week washout; sampling during weeks 1–8 and at week 12” (time origin: intervention_start); stated follow-up 3 mo.

Each row is one person (3 shown of 28); dots are samples at days since that person's first sample.

Capsule-preparation samples from 4 donors (comment1 Donor70/71/95/102) plus UC recipients sampled BL–W12; recipient–donor assignment not stated.

How longitudinal and relatedness information is derived

Community composition

Bacteroides 13.5%Faecalibacterium 11.5%Blautia_A 7.2%Roseburia 6.7%Collinsella 3.4%Bifidobacterium 3.3%Phocaeicola 3.2%Gemmiger 2.8%Parabacteroides 2.3%Mediterraneibacter 2.0%Fusicatenibacter 1.9%Alistipes 1.9%not assigned to a genus 6.0%other genera 34.3%

Genera, mean relative abundance over 183 profiled gut samples of this project (Sandpiper SingleM profiles, GTDB R232; low-depth and non-metagenome profiles left out).

Field coverage

field	samples	coverage	R1	R4
age_category	0	0%
country	188	100%	188
health_condition	188	100%		188
subject_id	188	100%	188
detailed_location	188	100%
health_condition_detail	188	100%		188
latitude	188	100%	188
location_locality	188	100%	188
location_region	188	100%	188
longitude	188	100%	188
timepoint_label	186	99%	186
intervention	103	55%	103

Papers (1)

Novel Microbial Engraftment Trajectories Following Microbiota Transplant Therapy in Ulcerative Colitis. 2025 PMID 39240145 PMC12476944 own_data

Details

Archive description

Double-blind, place-controlled study of intestinal microbiota transplantation to treat ulcerative colitis.

How this project entered the registry

Classified as human gut / stool shotgun DNA metagenome of age unknown by sonnet_x2 with confidence 0.75. Assay shotgun_dna.

host run.host_tax_id · “9606”
body site run.scientific_name · “human gut metagenome”

Evidence examples (one per field × route)

sample	field	value	route	conf.	source	quote
SAMN47133052	country	US	R1	0.90	sample.attr.geo_loc_name	USA: Minneapolis, MN
SAMN47133052	health_condition	ulcerative_colitis	R4	0.50	study.description	treat ulcerative colitis
SAMN47133052	health_condition_detail	Ulcerative colitis	R4	0.50	study.description	treat ulcerative colitis
SAMN47133054	intervention	fmt	R1	0.85	biosample.attribute:comment1	Donor71
SAMN47133107	intervention	fmt	R1	0.85	biosample.attribute:time_point	OLD0
SAMN47133052	latitude	44.9847	R1	0.90	biosample.attribute:lat_lon	44.9847 N 93.1819 W
SAMN47133052	location_locality	Minneapolis	R1	0.85	biosample.attribute:geo_loc_name	USA: Minneapolis, MN
SAMN47133052	location_region	Minnesota	R1	0.75	biosample.attribute:geo_loc_name	USA: Minneapolis, MN
SAMN47133052	longitude	-93.1819	R1	0.90	biosample.attribute:lat_lon	44.9847 N 93.1819 W
SAMN47133052	subject_id	NTC_Ctl_Vaughn_001	R1	0.85	sample.attr.participant_id	NTC_Ctl_Vaughn_001
SAMN47133054	timepoint_label	00017	R1	0.85	sample.attr.time_point	00017

Sequencing

188 runs over 188 samples · mean 8.71 Gbp per run (median 7.79 Gbp, total 1,637.08 Gbp; 188 runs with a base count) · layout PAIRED 188 · platform ILLUMINA 188 · models Illumina NovaSeq 6000 (188) · 97% of runs with a Sandpiper profile · first public 2025-03-02 – 2025-03-03

Authors (10)

Moutsoglou D first · Syal A · Lopez S · Nelson EC · Chen L · Kabage AJ · Fischer M · Khoruts A · Vaughn BP · Staley C last

Downloads

PRJNA844888.csv.gz (5.6 KB) · parquet (77.6 KB) — all 188 samples, every field with route and confidence · PRJNA844888_determinations.csv.gz (10.5 KB) — 1,793 evidence rows

Samples (188 · every field this project has values for · hover a cell for its source)

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