Gut metagenome in ITP patients vs healthy controls
Cross-sectional metagenomic study of 32 human gut samples comparing individuals with immune thrombocytopenia (ITP), an autoimmune/inflammatory condition, to healthy controls (16 vs 16), mostly adults, to characterize gut flora composition and function. machine-written summary
32 samples · 32 runs · CN · Adult · 2022 · 13.62 Gbp / sample
Checked this project's papers and archive records yourself? Mark it human-verified.
what each source gave
| ✓ Sequence archive | 128 sample values (age, health condition, health condition detail, sex) |
| – Abstract / description | no abstract or project description |
| – Full text | no open-access full text linked |
| – Supplementary tables | no supplementary tables available (or none open access) |
| ✓ External resources | 32 sample values (country) · from GMrepo v3 |
| – User contribution | nothing uploaded yet |
| – Human verified | not yet checked by a person · I checked this project — verify it |
Genera, mean relative abundance over 31 profiled gut samples of this project (Sandpiper SingleM profiles, GTDB R232; low-depth and non-metagenome profiles left out).
| field | samples | coverage | R1 | R2 | R3 | R4 |
|---|---|---|---|---|---|---|
| age_category | 32 | 100% | ||||
| country | 32 | 100% | 32 | |||
| health_condition | 32 | 100% | 32 | |||
| subject_id | 0 | 0% | ||||
| age_at_collection_days | 32 | 100% | 32 | |||
| health_condition_detail | 32 | 100% | 32 | |||
| sex | 32 | 100% | 32 |
- Intestinal flora altered and correlated with interleukin-2/4 in patients with primary immune thrombocytopenia. 2023 PMID 37921501 own_data
- Altered intestinal flora in patients with primary immune thrombocytopenia 2022 own_data
Archive description
Currently, only one study has been conducted on the gut flora of ITP patients using a metagenomics, and the evidence provided by the single-center study is limited due to the limited sample size of the study and the fact that gut flora is influenced by dietary, regional and genetic factors. Therefore, we will use metagenomic sequencing technology to further analyze the changes in the composition and function of intestinal flora in ITP patients, to further elucidate the role of gut flora in the etiology of ITP, and to provide biomarkers or potential diagnostic markers for ITP.
How this project entered the registry
Classified as human gut / stool shotgun DNA metagenome of adult by sonnet_x2 with confidence 0.62. Assay shotgun_dna.
- host run.scientific_name · “Homo sapiens”
- body site study.description · “gut flora of ITP patients”
- life stage sample.attr.age · “49 (n=30/32)”
Evidence examples (one per field × route)
| sample | field | value | route | conf. | source | quote |
|---|---|---|---|---|---|---|
| SAMN29663555 | age_at_collection_days | 22645.5 | R1 | 0.70 | sample.attr.age | 62 |
| SAMN29663555 | country | CN | R2 | 0.75 | external.gmrepo source | country: China |
| SAMN29663555 | health_condition | autoimmune_inflammatory | R1 | 0.70 | sample.name_rule:isolate | ITP |
| SAMN29663555 | health_condition_detail | ITP: The description states the study analyzes 'the gut flora of ITP patients' and 'the role of gut flora in the etiology of | R1 | 0.70 | sample.name_rule:isolate | ITP |
| SAMN29663555 | sex | female | R1 | 0.90 | sample.attr.sex | female |
Sequencing
32 runs over 32 samples · mean 13.77 Gbp per run (median 13.62 Gbp, total 440.52 Gbp; 32 runs with a base count) · layout PAIRED 32 · platform ILLUMINA 32 · models Illumina NovaSeq 6000 (32) · 97% of runs with a Sandpiper profile · first public 2022-07-16
Authors (0)
No author record.
Downloads
PRJNA858062.csv.gz (1.9 KB) · parquet (71.0 KB) — all 32 samples, every field with route and confidence · PRJNA858062_determinations.csv.gz (2.0 KB) — 160 evidence rows