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PRJNA866369 SRP390308

In vitro phage therapy on human fecal samples, Shenzhen

The metagenomic data of 18 samples of human feces with a 24-hours batch culture in vitro

18 human fecal samples underwent 24-hour in vitro batch culture in Shenzhen, China, comparing phage cocktail treatment versus heat-killed phage treatment, in an intervention study design. machine-written summary

18 samples · 18 runs · CN · Age unknown · 2023 · 0.95 Gbp / sample

Sources
✓Sequence archive 72–Abstract / description–Full text–Supplementary tables–External resources–User contribution–Human verified

Checked this project's papers and archive records yourself? Mark it human-verified.

what each source gave
✓ Sequence archive72 sample values (collection date, country, location locality, subject id)
– Abstract / descriptionno abstract or project description
– Full textno open-access full text linked
– Supplementary tablesno supplementary tables available (or none open access)
– External resourcesnot covered by the external resources we ingest
– User contributionnothing uploaded yet
– Human verifiednot yet checked by a person · I checked this project — verify it
Interventions

intervention — arms not resolved

Bacteriophage therapy design non_randomized_controlled · In vitro fecal batch culture with phage cocktail vs heat-killed phage

“under phage cocktail treatment or heat-killed phage treatment” — bioproject.description (study level; per-sample arms are in the intervention column below)

Samples at a glance
1 country
Age
unknown18
Country
CN18
Body site
primary18
Sequencing depth per sample · this project all projects
all projects, 0–0.03 Gbp: 25,080 samples (4 %)all projects, 0.03–0.1 Gbp: 17,066 samples (3 %)all projects, 0.1–0.3 Gbp: 29,179 samples (5 %)all projects, 0.3–1 Gbp: 47,044 samples (8 %)this project, 0.3–1 Gbp: 13 samplesall projects, 1–3 Gbp: 124,983 samples (22 %)this project, 1–3 Gbp: 5 samplesall projects, 3–10 Gbp: 253,728 samples (44 %)all projects, 10–30 Gbp: 72,533 samples (13 %)all projects, 30–100 Gbp: 3,024 samples (1 %)all projects, 100–300 Gbp: 106 samples (0 %)all projects, ≥ 300 Gbp: 9 samples (0 %)project median 0.95 Gbp per sample0130Gbp
Sampling design and relatedness

longitudinal — not resolved

3 of 3 people sampled more than once (18 samples; median 6, up to 6 per person).

How longitudinal and relatedness information is derived

Community composition
Blautia_A 11.5%Roseburia 11.0%Faecalibacterium 9.5%Anaerostipes 8.7%Phocaeicola 5.5%Bacteroides 4.8%Mediterraneibacter 4.8%Bifidobacterium 3.5%Dorea_A 3.1%Pilosibacter 3.0%Faecalibacillus 3.0%Prevotella 2.9%not assigned to a genus 8.6%other genera 20.0%

Genera, mean relative abundance over 16 profiled gut samples of this project (Sandpiper SingleM profiles, GTDB R232; low-depth and non-metagenome profiles left out).

Field coverage
fieldsamplescoverageR1R2R3R4
age_category0 0%
country18 100%18
health_condition0 0%
subject_id18 100%18
collection_date18 100%18
detailed_location18 100%
location_locality18 100%18
Details
Archive description

The raw sequencing reads of 18 samples of human feces with a 24-hours batch culture in vitro under phage cocktail treatment or heat-killed phage treatment.

How this project entered the registry

Classified as human gut / stool shotgun DNA metagenome of age unknown by sonnet_x2 with confidence 0.65. Assay shotgun_dna.

  • host run.scientific_name · “human gut metagenome”
  • body site study.title · “human feces with a 24-hours batch culture”
Evidence examples (one per field × route)
samplefieldvaluerouteconf.sourcequote
SAMN30175136collection_date2020R10.90biosample.attribute:collection_date2020
SAMN30175136countryCNR10.90sample.attr.geo_loc_nameChina:Shenzhen
SAMN30175136location_localityShenzhenR10.85biosample.attribute:geo_loc_nameChina:Shenzhen
SAMN30175136subject_idDonor1R10.70sample.name_rule:aliasDonor1_HKP_#1
Sequencing

18 runs over 18 samples · mean 0.94 Gbp per run (median 0.95 Gbp, total 16.91 Gbp; 18 runs with a base count) · layout PAIRED 18 · platform ILLUMINA 18 · models Illumina HiSeq 1500 (18) · 89% of runs with a Sandpiper profile · first public 2023-01-11

Authors (0)

No author record.

Downloads

PRJNA866369.csv.gz (1.2 KB) · parquet (69.4 KB) — all 18 samples, every field with route and confidence · PRJNA866369_determinations.csv.gz (1.0 KB) — 72 evidence rows

Samples (18 · every field this project has values for · hover a cell for its source)
Loading the sample table…