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PRJNA872328 SRP393854

Gut resistome in UK travellers to high-risk countries

GutBack

143 stool samples from adult travellers in the UK, collected pre-travel, post-travel, and at 6-month follow-up, to study antibiotic resistance gene carriage; longitudinal design. machine-written summary

143 samples · 143 runs · GB · Age unknown · 2022 · 4.50 Gbp / sample

Sources
✓Sequence archive 572✓Abstract / description 286○Full text○Supplementary tables–External resources–User contribution–Human verified

Checked this project's papers and archive records yourself? Mark it human-verified.

what each source gave
✓ Sequence archive572 sample values (collection date, country, subject id, timepoint)
✓ Abstract / description286 sample values (health condition, location locality)
○ Full textread; no usable statement or table · PMC10433802
○ Supplementary tablesfiles read; no table could be matched to the samples
– External resourcesnot covered by the external resources we ingest
– User contributionnothing uploaded yet
– Human verifiednot yet checked by a person · I checked this project — verify it
Samples at a glance
1 country
Age
adult143
Condition
healthy_control143
Country
GB143
Body site
primary143
Sequencing depth per sample · this project all projects
all projects, 0–0.03 Gbp: 25,080 samples (4 %)all projects, 0.03–0.1 Gbp: 17,066 samples (3 %)all projects, 0.1–0.3 Gbp: 29,179 samples (5 %)all projects, 0.3–1 Gbp: 47,044 samples (8 %)this project, 0.3–1 Gbp: 1 samplesall projects, 1–3 Gbp: 124,983 samples (22 %)this project, 1–3 Gbp: 15 samplesall projects, 3–10 Gbp: 253,728 samples (44 %)this project, 3–10 Gbp: 126 samplesall projects, 10–30 Gbp: 72,533 samples (13 %)this project, 10–30 Gbp: 1 samplesall projects, 30–100 Gbp: 3,024 samples (1 %)all projects, 100–300 Gbp: 106 samples (0 %)all projects, ≥ 300 Gbp: 9 samples (0 %)project median 4.50 Gbp per sample0130Gbp
Sampling design and relatedness

longitudinal — not resolved

48 of 48 people sampled more than once (143 samples; median 3, up to 3 per person). Schedule stated by the source: “Stool collected pre-travel (REP1), post-travel (REP2), and 6-month follow-up (REP3) per volunteer.” (time origin: other); stated follow-up 6 mo.

How longitudinal and relatedness information is derived

Community composition
Blautia_A 14.6%Bifidobacterium 8.3%Anaerobutyricum 5.4%Streptococcus 5.3%Anaerostipes 4.9%Romboutsia 3.8%Clostridium 3.6%Collinsella 3.6%Dorea_A 3.5%Mediterraneibacter 2.4%Faecalibacillus 2.4%Holdemanella 2.0%not assigned to a genus 6.1%other genera 34.0%

Genera, mean relative abundance over 129 profiled gut samples of this project (Sandpiper SingleM profiles, GTDB R232; low-depth and non-metagenome profiles left out).

Field coverage
fieldsamplescoverageR1R2R3R4
age_category143 100%
country143 100%143
health_condition143 100%143
subject_id143 100%143
collection_date143 100%143
detailed_location143 100%
location_locality143 100%143
timepoint_label143 100%143
Papers (1)
  • Prevalence and Persistence of Antibiotic Resistance Determinants in the Gut of Travelers Returning to the United Kingdom is Associated with Colonization by Pathogenic Escherichia coli. 2023 PMID 37255437 PMC10433802 own_data
Details
Archive description

Prevalence and persistence of antibiotic resistance determinants in the gut of travellers returning to the UK - a metagenomics study

How this project entered the registry

Classified as human gut / stool shotgun DNA metagenome of age unknown by sonnet_x2 with confidence 0.57. Assay shotgun_dna.

  • host run.host_tax_id · “9606”
  • host run.scientific_name · “gut metagenome”
  • body site study.description · “antibiotic resistance determinants in the gut”
Evidence examples (one per field × route)
samplefieldvaluerouteconf.sourcequote
SAMN30455338collection_date2016R10.90biosample.attribute:collection_date2016
SAMN30455338countryGBR10.90sample.attr.geo_loc_nameUnited Kingdom
SAMN30455338health_conditionhealthy_controlR40.40paper.abstractFifty volunteers, recruited at a travel clinic
SAMN30455338location_localityLondonR40.50paper.abstractrecruited at a travel clinic in London
SAMN30455338subject_idGUT042R10.70sample.name_rule:source_material_idGUT042_REP2
SAMN30455338timepoint_labelREP2R10.70sample.name_rule:source_material_idGUT042_REP2
Sequencing

143 runs over 143 samples · mean 4.56 Gbp per run (median 4.50 Gbp, total 652.09 Gbp; 143 runs with a base count) · layout PAIRED 143 · platform ILLUMINA 143 · models Illumina HiSeq 2500 (143) · 90% of runs with a Sandpiper profile · first public 2022-08-25 – 2022-08-26

Authors (12)

Dallman TJ first · Neuert S · Fernandez Turienzo C · Berin M · Richardson E · Fuentes-Utrilla P · Loman N · Gharbia S · Jenkins C · Behrens RH · Godbole G · Brown M last

Downloads

PRJNA872328.csv.gz (4.4 KB) · parquet (74.6 KB) — all 143 samples, every field with route and confidence · PRJNA872328_determinations.csv.gz (6.8 KB) — 858 evidence rows

Samples (143 · every field this project has values for · hover a cell for its source)
Loading the sample table…