Gut microbiota in adults under environmental stress
60 fecal samples from healthy adult subjects exposed to moderate and extreme stressful environmental conditions, collected at different time points in a longitudinal shotgun metagenome study. machine-written summary
60 samples · 60 runs · Adult · 2023 · 4.92 Gbp / sample
Checked this project's papers and archive records yourself? Mark it human-verified.
what each source gave
| ✓ Sequence archive | 360 sample values (age, lifestyle, sex, shared setting id, shared setting type, subject id, timepoint) |
| ✓ Abstract / description | 60 sample values (health condition) |
| – Full text | no open-access full text linked |
| – Supplementary tables | no supplementary tables available (or none open access) |
| – External resources | not covered by the external resources we ingest |
| – User contribution | nothing uploaded yet |
| – Human verified | not yet checked by a person · I checked this project — verify it |
longitudinal — not resolvedworkplace
20 of 20 people sampled more than once (60 samples; median 3, up to 3 per person). Schedule stated by the source: “Samples collected at multiple time points (T1, T2, T3) per subject under stress exposure conditions.” (time origin: other: stress exposure start).
10 members of the 38th (ISEA) Expedition (E01–E10, 3 time points) share the expedition; 10 moderate-stress subjects (D) have no stated unit1 shared settings.
member · circles are people (pale = no sequenced sample), arrows run donor → recipient and parent → child, curved lines join twins, partners and siblings, squares are shared settings or families without recorded roles. Shown: 1 shared setting. 20 people in the project overall.
Genera, mean relative abundance over 53 profiled gut samples of this project (Sandpiper SingleM profiles, GTDB R232; low-depth and non-metagenome profiles left out).
| field | samples | coverage | R1 | R2 | R3 | R4 |
|---|---|---|---|---|---|---|
| age_category | 60 | 100% | ||||
| country | 0 | 0% | ||||
| health_condition | 60 | 100% | 60 | |||
| subject_id | 60 | 100% | 60 | |||
| age_at_collection_days | 60 | 100% | 60 | |||
| lifestyle | 60 | 100% | 60 | |||
| sex | 60 | 100% | 60 | |||
| timepoint_label | 60 | 100% | 60 | |||
| shared_setting_id | 30 | 50% | 30 | |||
| shared_setting_type | 30 | 50% | 30 |
Archive description
Fecal samples were collected from healthy human subjects exposed to moderate and extreme stressful environmental conditions, to study the gut microbial changes. The samples were collected at different time points. Samples were analyzed for shotgun sequencing metagenome.
How this project entered the registry
Classified as human gut / stool shotgun DNA metagenome of adult by sonnet_x2 with confidence 0.80. Assay shotgun_dna.
- host study.description · “healthy human subjects exposed to moderate”
- body site study.description · “Fecal samples were collected”
- life stage sample.attr.age · “28 (n=60/60)”
Evidence examples (one per field × route)
| sample | field | value | route | conf. | source | quote |
|---|---|---|---|---|---|---|
| SAMN30672186 | age_at_collection_days | 15330.5 | R1 | 0.85 | sample.attr.age | 42 |
| SAMN30672186 | health_condition | healthy_control | R4 | 0.50 | study.description | healthy human subjects |
| SAMN30672186 | lifestyle | extreme_environment | R1 | 0.85 | biosample.attribute:isolate | 38th (ISEA) Expedition members |
| SAMN30672186 | sex | male | R1 | 0.90 | sample.attr.sex | male |
| SAMN30672186 | shared_setting_id | 38th (ISEA) Expedition | R1 | 0.85 | biosample.attribute:isolate | 38th (ISEA) Expedition members |
| SAMN30672186 | shared_setting_type | workplace | R1 | 0.85 | biosample.attribute:isolate | 38th (ISEA) Expedition members |
| SAMN30672186 | subject_id | E02 | R1 | 0.70 | sample.name_rule:library_names | E02-T1 |
| SAMN30672186 | timepoint_label | T1 | R1 | 0.70 | sample.name_rule:library_names | E02-T1 |
Sequencing
60 runs over 60 samples · mean 4.64 Gbp per run (median 4.92 Gbp, total 278.69 Gbp; 60 runs with a base count) · layout PAIRED 60 · platform ILLUMINA 60 · models HiSeq X Ten (60) · 88% of runs with a Sandpiper profile · first public 2023-10-02
Authors (0)
No author record.
Downloads
PRJNA876774.csv.gz (2.5 KB) · parquet (71.8 KB) — all 60 samples, every field with route and confidence · PRJNA876774_determinations.csv.gz (3.8 KB) — 420 evidence rows