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PRJNA885289 SRP402082

Microbiome in esports players vs athletes & students, Poland

Characteristics of the gut microbiome in esports players compared with those in physical education students and professional athletes

169 fecal samples from Polish male esports players, endurance athletes, and physical education students (Warsaw, Cracow), comparing gut microbiota composition, function, and metabolites in a case-control design. machine-written summary

169 samples · 169 runs · PL · Age unknown · 2023 · 4.35 Gbp / sample · cohort Microbial and Metabolic Gut Profiling across Seven

Sources
✓Sequence archive 870✓Abstract / description 169✓Full text 169○Supplementary tables–External resources–User contribution–Human verified

Checked this project's papers and archive records yourself? Mark it human-verified.

what each source gave
✓ Sequence archive870 sample values (collection date, country, latitude, location locality, location region, longitude)
✓ Abstract / description169 sample values (health condition detail)
✓ Full text169 sample values (antibiotic exposure) · PMC12298630, PMC9884692
○ Supplementary tablesfiles read; no table could be matched to the samples
– External resourcesnot covered by the external resources we ingest
– User contributionnothing uploaded yet · share metadata
– Human verifiednot yet checked by a person · I checked this project — verify it
Samples at a glance
2 sampling locations · 1 country
Age
unknown169
Country
PL169
Body site
primary169
Sequencing depth per sample · this project all projects
all projects, 0–0.03 Gbp: 25,080 samples (4 %)all projects, 0.03–0.1 Gbp: 17,066 samples (3 %)all projects, 0.1–0.3 Gbp: 29,179 samples (5 %)all projects, 0.3–1 Gbp: 47,044 samples (8 %)all projects, 1–3 Gbp: 124,983 samples (22 %)this project, 1–3 Gbp: 61 samplesall projects, 3–10 Gbp: 253,728 samples (44 %)this project, 3–10 Gbp: 106 samplesall projects, 10–30 Gbp: 72,533 samples (13 %)this project, 10–30 Gbp: 2 samplesall projects, 30–100 Gbp: 3,024 samples (1 %)all projects, 100–300 Gbp: 106 samples (0 %)all projects, ≥ 300 Gbp: 9 samples (0 %)project median 4.35 Gbp per sample0130Gbp
Community composition
Bacteroides 16.5%Alistipes 15.2%Phocaeicola 10.6%Prevotella 7.2%Faecalibacterium 3.8%Hominicoprocola 2.4%Gemmiger 2.1%Sutterella 2.0%Parabacteroides 1.5%Vescimonas 1.5%Roseburia 1.4%UBA11524 1.3%not assigned to a genus 6.4%other genera 28.0%

Genera, mean relative abundance over 158 profiled gut samples of this project (Sandpiper SingleM profiles, GTDB R232; low-depth and non-metagenome profiles left out).

Field coverage
fieldsamplescoverageR1R2R3R4
age_category0 0%
country169 100%169
health_condition0 0%
subject_id0 0%
antibiotic_exposure169 100%169
detailed_location169 100%
health_condition_detail169 100%169
latitude169 100%169
location_locality169 100%169
longitude169 100%169
collection_date144 85%144
location_region50 30%50
Papers (3)
  • Characteristics of the gut microbiome in esports players compared with those in physical education students and professional athletes. 2022 PMID 36726816 PMC9884692 own_data
  • The Gut Microbiome Obesity Index: A New Analytical Tool in the Metagenomics Workflow for the Evaluation of Gut Dysbiosis in Obese Humans. 2025 PMID 40732945 PMC12298630 own_data
  • Microbial and Metabolic Gut Profiling across Seven Malignancies Identifies Fecal Faecalibacillus intestinalis and Formic Acid as Commonly Altered in Cancer Patients. 2024 PMID 39125593 PMC11311272 unsure
Details
Archive description

Esports is a category of competitive video games that, in many aspects, may be similar to traditional sports however, the gut microbiota composition of esport players has not been yet studied.</p><p>Here, we investigated the composition and function of the gut microbiota, as well as bacterial metabolites, short chain fatty acids (SCFAs), and amino acids, in a group of 109 well-characterized Polish male esports players. The results were compared with two reference groups: 25 endurance athletes and 36 healthy students of physical education. DNA and metabolites isolated from fecal samples were analyzed using shotgun metagenomic sequencing and mass spectrometry, respectively. Physical activity and nutritional measures were evaluated by questionnaire.

How this project entered the registry

Classified as human gut / stool shotgun DNA metagenome of age unknown by opus_adjudicated with confidence 0.70. Assay shotgun_dna.

  • host run.host_tax_id · “9606”
  • host study.description · “109 well-characterized Polish male esports players”
  • body site study.description · “DNA and metabolites isolated from fecal samples”
Evidence examples (one per field × route)
samplefieldvaluerouteconf.sourcequote
SAMN31248723antibiotic_exposurenoR30.70paper.fulltext.methodsNone of the participants had used antibiotics in the 2 months
SAMN31248723collection_date2020-07-28R10.90biosample.attribute:collection_date2020-07-28
SAMN31248723countryPLR10.90sample.attr.geo_loc_namePoland:Warsaw
SAMN31248723health_condition_detailmixed_case_control: esports players vs endurance athletes vs healthy physical education studentsR40.40study.description109 well-characterized Polish male esports players. The results were compared with two
SAMN31248723latitude52.1400R10.90biosample.attribute:lat_lon52.14 N 21 E
SAMN31248723location_localityWarszawaR10.75biosample.attribute:geo_loc_namePoland:Warsaw
SAMN31248783location_regionLesser Poland VoivodeshipR10.60biosample.attribute:lat_lon50.03 N 19.56 E
SAMN31248723longitude21.0000R10.90biosample.attribute:lat_lon52.14 N 21 E
Sequencing

169 runs over 169 samples · mean 4.35 Gbp per run (median 4.35 Gbp, total 735.83 Gbp; 169 runs with a base count) · layout PAIRED 169 · platform ILLUMINA 169 · models Illumina NovaSeq 6000 (169) · 93% of runs with a Sandpiper profile · first public 2023-01-08

Authors (36)

Kulecka M first · Fraczek B · Balabas A · Czarnowski P · Zeber-Lubecka N · Zapala B · Baginska K · Glowienka M · Szot M · Skorko M · Kluska A · Piatkowska M · Mikula M · Ostrowski J last · Bałabas A · Turkot M · Kruczkowska-Tarantowicz K · Żeber-Lubecka N · Dąbrowska M · Paszkiewicz-Kozik E · Walewski J · Ługowska I · Koseła-Paterczyk H · Rutkowski P · Piątkowska M · Jagiełło-Gruszfeld A · Tenderenda M · Gawiński C · Wyrwicz L · Borucka M · Krzakowski M · Zając L · Kamiński M · Jaworski P · Frączek B · Tarnowski W

Help complete this project

3 of the 4 core fields are below 50% coverage: age_category, health_condition, subject_id. Share metadata

Downloads

PRJNA885289.csv.gz (5.2 KB) · parquet (77.0 KB) — all 169 samples, every field with route and confidence · PRJNA885289_determinations.csv.gz (7.8 KB) — 1,208 evidence rows

Samples (169 · every field this project has values for · hover a cell for its source)
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