GutAlive vs Zymo Shield stool kit, Asturias, Spain
32 fecal samples from 5 healthy adult donors in Asturias, Spain, comparing GutAlive and Zymo DNA/RNA Shield collection devices across four processing time points using shotgun metagenomics. machine-written summary
32 samples · 32 runs · ES · Age unknown · 2023 · 4.08 Gbp / sample
Checked this project's papers and archive records yourself? Mark it human-verified.
what each source gave
| ✓ Sequence archive | 224 sample values (collection date, country, latitude, location region, longitude, subject id, timepoint) |
| ○ Abstract / description | read; no usable statement |
| ✓ Full text | 128 sample values (antibiotic exposure, health condition, health condition detail, location site) · PMC10117842 |
| ○ Supplementary tables | files read; no table could be matched to the samples |
| – External resources | not covered by the external resources we ingest |
| – User contribution | nothing uploaded yet |
| – Human verified | not yet checked by a person · I checked this project — verify it |
longitudinal — not resolved
4 of 5 people sampled more than once (31 samples; median 8, up to 8 per person).
Genera, mean relative abundance over 32 profiled gut samples of this project (Sandpiper SingleM profiles, GTDB R232; low-depth and non-metagenome profiles left out).
| field | samples | coverage | R1 | R2 | R3 | R4 |
|---|---|---|---|---|---|---|
| age_category | 32 | 100% | ||||
| country | 32 | 100% | 32 | |||
| health_condition | 32 | 100% | 32 | |||
| subject_id | 32 | 100% | 32 | |||
| antibiotic_exposure | 32 | 100% | 32 | |||
| collection_date | 32 | 100% | 32 | |||
| detailed_location | 32 | 100% | ||||
| health_condition_detail | 32 | 100% | 32 | |||
| latitude | 32 | 100% | 32 | |||
| location_region | 32 | 100% | 32 | |||
| location_site | 32 | 100% | 32 | |||
| longitude | 32 | 100% | 32 | |||
| timepoint_label | 32 | 100% | 32 |
- GutAlive® enables DNA-based microbiome analysis without disrupting the original composition and diversity. 2023 PMID 37089545 PMC10117842 own_data
Archive description
GutAlive enables microbiome analysis overtime
How this project entered the registry
Classified as human gut / stool shotgun DNA metagenome of age unknown by sonnet_x2 with confidence 0.65. Assay shotgun_dna.
- host run.host_tax_id · “9606”
- body site run.scientific_name · “human gut metagenome”
Evidence examples (one per field × route)
| sample | field | value | route | conf. | source | quote |
|---|---|---|---|---|---|---|
| SAMN32064825 | antibiotic_exposure | no | R3 | 0.70 | paper.fulltext.methods | antibiotics or glucocorticoids in the previous 3 months |
| SAMN32064825 | collection_date | 2022 | R1 | 0.90 | biosample.attribute:collection_date | 2022 |
| SAMN32064825 | country | ES | R1 | 0.90 | sample.attr.geo_loc_name | Spain: Asturias |
| SAMN32064825 | health_condition | healthy_control | R3 | 0.70 | paper.fulltext.methods | fecal samples from four healthy donors |
| SAMN32064825 | health_condition_detail | healthy donors | R3 | 0.70 | paper.fulltext.methods | fecal samples from four healthy donors |
| SAMN32064825 | latitude | 43.3546 | R1 | 0.90 | biosample.attribute:lat_lon | 43.35462849 N 5.87166109 W |
| SAMN32064825 | location_region | Asturias | R1 | 0.85 | biosample.attribute:geo_loc_name | Spain: Asturias |
| SAMN32064825 | location_site | Microviable Therapeutics SL | R3 | 0.70 | paper.fulltext.methods | carried out at Microviable Therapeutics SL (Asturias, Spain) |
| SAMN32064825 | longitude | -5.8717 | R1 | 0.90 | biosample.attribute:lat_lon | 43.35462849 N 5.87166109 W |
| SAMN32064825 | subject_id | 1 | R1 | 0.50 | sample.attr.donor | 1 |
| SAMN32064825 | timepoint_label | 24 | R1 | 0.85 | biosample.attribute:time_hours | time_hours: 24 |
Sequencing
32 runs over 32 samples · mean 4.26 Gbp per run (median 4.08 Gbp, total 136.41 Gbp; 32 runs with a base count) · layout PAIRED 32 · platform ILLUMINA 32 · models Illumina NovaSeq 6000 (32) · 100% of runs with a Sandpiper profile · first public 2023-10-28
Authors (4)
Montero I first · Barrientos D · Hidalgo-Cantabrana C · Martínez-Álvarez N last
Downloads
PRJNA909041.csv.gz (1.7 KB) · parquet (71.5 KB) — all 32 samples, every field with route and confidence · PRJNA909041_determinations.csv.gz (2.9 KB) — 352 evidence rows