Gut Microbiome Nanopore Sequencing in Healthy Adults
Cross-sectional study of 2 gut microbiome samples from healthy adult controls in Wisconsin, USA, using Nanopore sequencing to survey intestinal microbial community structure. machine-written summary
2 samples · 2 runs · US · Adult · 2023 · 0 / sample
Checked this project's papers and archive records yourself? Mark it human-verified.
what each source gave
| ✓ Sequence archive | 24 sample values (age, bmi, collection date, country, diet, diet detail, health condition detail, latitude) |
| ✓ Abstract / description | 2 sample values (health condition) |
| – Full text | no open-access full text linked |
| – Supplementary tables | no supplementary tables available (or none open access) |
| – External resources | not covered by the external resources we ingest |
| – User contribution | nothing uploaded yet |
| – Human verified | not yet checked by a person · I checked this project — verify it |
No Sandpiper profile for this project's samples yet.
| field | samples | coverage | R1 | R2 | R3 | R4 |
|---|---|---|---|---|---|---|
| age_category | 2 | 100% | ||||
| country | 2 | 100% | 2 | |||
| health_condition | 2 | 100% | 2 | |||
| subject_id | 2 | 100% | 2 | |||
| age_at_collection_days | 2 | 100% | 2 | |||
| bmi | 2 | 100% | 2 | |||
| collection_date | 2 | 100% | 2 | |||
| detailed_location | 2 | 100% | ||||
| diet | 2 | 100% | 2 | |||
| diet_detail | 2 | 100% | 2 | |||
| health_condition_detail | 2 | 100% | 2 | |||
| latitude | 2 | 100% | 2 | |||
| location_region | 2 | 100% | 2 | |||
| longitude | 2 | 100% | 2 | |||
| sex | 2 | 100% | 2 |
Archive description
The microbiome -- the commensal microbes that colonize the human skin, mouth, gut, and urinogenital tract -- play an important but poorly characterized role in human health and disease. Microbial communities in the gut are of particular interest: they impact not only digestion, but also our immune systems and even our mental health! Part of the challenge in characterizing this role is the immense diversity of these microbial communities -- the microbes present in an individual's large intestine seem to be impacted by heredity, environment, diet, disease, and other factors. Thus, simply surveying these communities from healthy individuals to determine which microbes are present, and in what proportions, provides useful data as to community structure and may allow us to infer the metabolic activity of these communities. Ultimately, understanding the structure and function of these commensal microbial communities may allow us to alter them rationally, leading to better health outcomes.
How this project entered the registry
Classified as human gut / stool shotgun DNA metagenome of adult by sonnet_x2 with confidence 0.55. Assay shotgun_dna.
- host run.host_tax_id · “9606”
- body site run.scientific_name · “gut metagenome”
- life stage study.description · “surveying these communities from healthy individuals”
Evidence examples (one per field × route)
| sample | field | value | route | conf. | source | quote |
|---|---|---|---|---|---|---|
| SAMN32192830 | age_at_collection_days | 9496.5 | R1 | 0.85 | sample.attr.host_age | 26 |
| SAMN32192830 | bmi | 23.4 | R1 | 0.90 | sample.attr.host_body_mass_index | 23.4 |
| SAMN32192830 | collection_date | 2022-11-09 | R1 | 0.90 | biosample.attribute:collection_date | 2022-11-09 |
| SAMN32192830 | country | US | R1 | 0.90 | sample.attr.geo_loc_name | USA:Wisconsin |
| SAMN32192830 | diet | omnivore | R1 | 0.90 | biosample.attribute:host_diet | Omnivore |
| SAMN32192830 | diet_detail | Omnivore | R1 | 0.90 | biosample.attribute:host_diet | Omnivore |
| SAMN32192830 | health_condition | healthy_control | R4 | 0.35 | study.description | surveying these communities from healthy individuals |
| SAMN32192830 | health_condition_detail | Caucasian, brown hair, green eyes. | R1 | 0.90 | sample.attr.host_phenotype | Caucasian, brown hair, green eyes. |
| SAMN32192830 | latitude | 44.8700 | R1 | 0.90 | biosample.attribute:lat_lon | 44.87 N 91.93 W |
| SAMN32192830 | location_region | Wisconsin | R1 | 0.85 | biosample.attribute:geo_loc_name | USA:Wisconsin |
| SAMN32192830 | longitude | -91.9300 | R1 | 0.90 | biosample.attribute:lat_lon | 44.87 N 91.93 W |
| SAMN32192830 | sex | female | R1 | 0.90 | sample.attr.host_sex | female |
| SAMN32192830 | subject_id | 1 | R1 | 0.85 | sample.attr.host_subject_id | 1 |
Sequencing
2 runs over 2 samples · mean 0 per run (median 0, total 0; 2 runs with a base count) · layout SINGLE 2 · platform OXFORD_NANOPORE 2 · models MinION (2) · 0% of runs with a Sandpiper profile · first public 2023-01-26
Authors (0)
No author record.
Downloads
PRJNA911758.csv.gz (989 B) · parquet (70.8 KB) — all 2 samples, every field with route and confidence · PRJNA911758_determinations.csv.gz (1.1 KB) — 26 evidence rows