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PRJNA911758 SRP412828

Gut Microbiome Nanopore Sequencing in Healthy Adults

Studying Intestinal Microbiomes by Nanopore Sequencing

Cross-sectional study of 2 gut microbiome samples from healthy adult controls in Wisconsin, USA, using Nanopore sequencing to survey intestinal microbial community structure. machine-written summary

2 samples · 2 runs · US · Adult · 2023 · 0 / sample

Sources
✓Sequence archive 24✓Abstract / description 2–Full text–Supplementary tables–External resources–User contribution–Human verified

Checked this project's papers and archive records yourself? Mark it human-verified.

what each source gave
✓ Sequence archive24 sample values (age, bmi, collection date, country, diet, diet detail, health condition detail, latitude)
✓ Abstract / description2 sample values (health condition)
– Full textno open-access full text linked
– Supplementary tablesno supplementary tables available (or none open access)
– External resourcesnot covered by the external resources we ingest
– User contributionnothing uploaded yet
– Human verifiednot yet checked by a person · I checked this project — verify it
Samples at a glance
1 sampling locations · 1 country
Age
adult2
Condition
healthy_control2
Sex
female1
male1
Country
US2
Body site
primary2
Community composition

No Sandpiper profile for this project's samples yet.

Field coverage
fieldsamplescoverageR1R2R3R4
age_category2 100%
country2 100%2
health_condition2 100%2
subject_id2 100%2
age_at_collection_days2 100%2
bmi2 100%2
collection_date2 100%2
detailed_location2 100%
diet2 100%2
diet_detail2 100%2
health_condition_detail2 100%2
latitude2 100%2
location_region2 100%2
longitude2 100%2
sex2 100%2
Details
Archive description

The microbiome -- the commensal microbes that colonize the human skin, mouth, gut, and urinogenital tract -- play an important but poorly characterized role in human health and disease. Microbial communities in the gut are of particular interest: they impact not only digestion, but also our immune systems and even our mental health! Part of the challenge in characterizing this role is the immense diversity of these microbial communities -- the microbes present in an individual's large intestine seem to be impacted by heredity, environment, diet, disease, and other factors. Thus, simply surveying these communities from healthy individuals to determine which microbes are present, and in what proportions, provides useful data as to community structure and may allow us to infer the metabolic activity of these communities. Ultimately, understanding the structure and function of these commensal microbial communities may allow us to alter them rationally, leading to better health outcomes.

How this project entered the registry

Classified as human gut / stool shotgun DNA metagenome of adult by sonnet_x2 with confidence 0.55. Assay shotgun_dna.

  • host run.host_tax_id · “9606”
  • body site run.scientific_name · “gut metagenome”
  • life stage study.description · “surveying these communities from healthy individuals”
Evidence examples (one per field × route)
samplefieldvaluerouteconf.sourcequote
SAMN32192830age_at_collection_days9496.5R10.85sample.attr.host_age26
SAMN32192830bmi23.4R10.90sample.attr.host_body_mass_index23.4
SAMN32192830collection_date2022-11-09R10.90biosample.attribute:collection_date2022-11-09
SAMN32192830countryUSR10.90sample.attr.geo_loc_nameUSA:Wisconsin
SAMN32192830dietomnivoreR10.90biosample.attribute:host_dietOmnivore
SAMN32192830diet_detailOmnivoreR10.90biosample.attribute:host_dietOmnivore
SAMN32192830health_conditionhealthy_controlR40.35study.descriptionsurveying these communities from healthy individuals
SAMN32192830health_condition_detailCaucasian, brown hair, green eyes.R10.90sample.attr.host_phenotypeCaucasian, brown hair, green eyes.
SAMN32192830latitude44.8700R10.90biosample.attribute:lat_lon44.87 N 91.93 W
SAMN32192830location_regionWisconsinR10.85biosample.attribute:geo_loc_nameUSA:Wisconsin
SAMN32192830longitude-91.9300R10.90biosample.attribute:lat_lon44.87 N 91.93 W
SAMN32192830sexfemaleR10.90sample.attr.host_sexfemale
SAMN32192830subject_id1R10.85sample.attr.host_subject_id1
Sequencing

2 runs over 2 samples · mean 0 per run (median 0, total 0; 2 runs with a base count) · layout SINGLE 2 · platform OXFORD_NANOPORE 2 · models MinION (2) · 0% of runs with a Sandpiper profile · first public 2023-01-26

Authors (0)

No author record.

Downloads

PRJNA911758.csv.gz (989 B) · parquet (70.8 KB) — all 2 samples, every field with route and confidence · PRJNA911758_determinations.csv.gz (1.1 KB) — 26 evidence rows

Samples (2 · every field this project has values for · hover a cell for its source)
Loading the sample table…