Gut microbiota in melanoma with FMT + anti-PD1 (Canada)
71 fecal samples from patients with advanced melanoma (plus healthy controls) in Montreal and London, Canada, profiled by 16S rRNA sequencing across four time points around fecal microbiota transplant combined with anti-PD1 immunotherapy. machine-written summary
71 samples · 71 runs · CA · Adult · 2023 · 5.20 Gbp / sample
Checked this project's papers and archive records yourself? Mark it human-verified.
what each source gave
| ✓ Sequence archive | 879 sample values (age, bmi, collection date, country, health condition, health condition detail, latitude, location locality) |
| ○ Abstract / description | read; no usable statement |
| ○ Full text | read; no usable statement or table · PMC11629153, PMC12359429, PMC12390848, PMC12590682, PMC13173278 |
| ○ Supplementary tables | files read; no table could be matched to the samples |
| – External resources | not covered by the external resources we ingest |
| – User contribution | nothing uploaded yet |
| – Human verified | not yet checked by a person · I checked this project — verify it |
intervention — arms not resolved
Non-antibiotic pharmaceutical given as the study intervention (metformin Faecal microbiota transplantation design single_arm_before_after · population other_cancer · FMT from healthy donor combined with anti-PD1 therapy in melanoma
“single FMT with healthy donor stool followed by standard anti-PD1 therapy” — abstract:40759441 (study level; per-sample arms are in the intervention column below)
longitudinal — not resolvedFMT donor → recipient
19 of 21 people sampled more than once (69 samples; median 4, up to 5 per person). Schedule stated by the source: “Four time points: preFMT/preICI (baseline), postFMT (7 days)/preICI, postFMT/postICI (3 weeks), postFMT/postICI (3 months).” (time origin: transplant); stated follow-up 3 mo.
3 healthy FMT donors (D1, D2, D5) sequenced; 11 donor stool samples each titled with the recipient patient id (11 donor->recipient links).
donorrecipient · circles are people (pale = no sequenced sample), arrows run donor → recipient and parent → child, curved lines join twins, partners and siblings, squares are shared settings or families without recorded roles. Shown: 3 linked groups from explicit relations. 21 people in the project overall.
Genera, mean relative abundance over 65 profiled gut samples of this project (Sandpiper SingleM profiles, GTDB R232; low-depth and non-metagenome profiles left out).
| field | samples | coverage | R1 | R2 | R3 | R4 |
|---|---|---|---|---|---|---|
| age_category | 71 | 100% | ||||
| country | 71 | 100% | 71 | |||
| health_condition | 71 | 100% | 71 | |||
| subject_id | 71 | 100% | 71 | |||
| collection_date | 71 | 100% | 71 | |||
| detailed_location | 71 | 100% | ||||
| health_condition_detail | 71 | 100% | 71 | |||
| latitude | 71 | 100% | 71 | |||
| location_locality | 71 | 100% | 71 | |||
| location_region | 71 | 100% | 71 | |||
| longitude | 71 | 100% | 71 | |||
| age_at_collection_days | 60 | 85% | 60 | |||
| bmi | 60 | 85% | 60 | |||
| sex | 60 | 85% | 60 | |||
| timepoint_label | 60 | 85% | 60 |
- Improved survival in advanced melanoma patients treated with fecal microbiota transplantation using healthy donor stool in combination with anti-PD1: final results of the MIMic phase 1 trial. 2025 PMID 40759441 PMC12359429 own_data
- Longitudinal analysis of the gut microbiota during anti-PD-1 therapy reveals stable microbial features of response in melanoma patients. 2024 PMID 39481388 PMC11629153 unsure
- Microbiota-driven antitumour immunity mediated by dendritic cell migration. 2025 PMID 40659786 PMC12390848 unsure
- Accurate profiling of microbial communities for shotgun metagenomic sequencing with Meteor2. 2025 PMID 41199348 PMC12590682 unsure
- Ecological and Functional Stratification of the Stool Microbiome Predicts Response to Immune Checkpoint Inhibitors across Cancer Types. 2026 PMID 42146906 PMC13173278 unsure
Archive description
Bacterial communities of patient fecal samples were profiled via V3-V4 region 16S rRNA gene sequencing at four time points: i) prior to both fecal microbiota transplant (FMT) and anti-PD-1 immune checkpoint inhibitor (ICI) therapy, ii) post-FMT (7 days) / pre-ICI, iii) post-FMT / post-ICI (3 weeks), and iv) post-FMT / post-ICI (3 months)
How this project entered the registry
Classified as human gut / stool shotgun DNA metagenome of adult and elderly by deterministic_prior with confidence 0.90. Assay shotgun_dna.
- host external_curation.infant_catalog · “infant triage reason_code age_adult_only”
- body site study.title · “Gut microbiota composition of”
- body site study.description · “communities of patient fecal samples were profiled”
- life stage external_curation.infant_catalog · “infant triage reason_code age_adult_only”
- life stage sample.attr.host_age · “82 (n=43/71)”
Evidence examples (one per field × route)
| sample | field | value | route | conf. | source | quote |
|---|---|---|---|---|---|---|
| SAMN32941640 | age_at_collection_days | 24837.0 | R1 | 0.70 | sample.attr.host_age | 68 |
| SAMN32941640 | bmi | 27.48 | R1 | 0.90 | sample.attr.host_body_mass_index | 27.47562949 |
| SAMN32941640 | collection_date | 2023-01-20 | R1 | 0.90 | biosample.attribute:collection_date | 2023-01-20 |
| SAMN32941640 | country | CA | R1 | 0.90 | sample.attr.geo_loc_name | Canada: London |
| SAMN32941640 | health_condition | other_cancer | R1 | 0.85 | biosample_attr | melanoma_M1b |
| SAMN32941640 | health_condition_detail | melanoma_M1b | R1 | 0.90 | sample.attr.host_disease | melanoma_M1b |
| SAMN32941640 | latitude | 42.9600 | R1 | 0.90 | biosample.attribute:lat_lon | 42.96 N 81.23 W |
| SAMN32941640 | location_locality | London | R1 | 0.85 | biosample.attribute:geo_loc_name | Canada: London |
| SAMN32941640 | location_region | Ontario | R1 | 0.60 | biosample.attribute:lat_lon | 42.96 N 81.23 W |
| SAMN32941640 | longitude | -81.2300 | R1 | 0.90 | biosample.attribute:lat_lon | 42.96 N 81.23 W |
| SAMN32941640 | sex | female | R1 | 0.90 | sample.attr.host_sex | female |
| SAMN32941640 | subject_id | L623_P3 | R1 | 0.85 | sample.attr.host_subject_id | L623_P3 |
| SAMN32941640 | timepoint_label | preFMT_preICI | R1 | 0.70 | sample.name_rule:description | L623_P3_S1_preFMT_preICI |
Sequencing
71 runs over 71 samples · mean 6.59 Gbp per run (median 5.20 Gbp, total 467.68 Gbp; 71 runs with a base count) · layout PAIRED 71 · platform ILLUMINA 71 · models Illumina NovaSeq 6000 (71) · 92% of runs with a Sandpiper profile · first public 2023-02-09
Authors (94)
Macandog ADG first · Catozzi C · Capone M · Nabinejad A · Nanaware PP · Liu S · Vinjamuri S · Stunnenberg JA · Galiè S · Jodice MG · Montani F · Armanini F · Cassano E · Madonna G · Mallardo D · Mazzi B · Pece S · Tagliamonte M · Vanella V · Barberis M · Ferrucci PF · Blank CU · Bouvier M · Andrews MC · Xu X · Santambrogio L · Segata N · Buonaguro L · Cocorocchio E · Ascierto PA · Manzo T · Nezi L last · Lin NY first · Fukuoka S · Koyama S · Motooka D · Tourlousse DM · Shigeno Y · Matsumoto Y · Yamano H · Murotomi K · Tamaki H · Irie T · Sugiyama E · Kumagai S · Itahashi K · Tanegashima T · Fujimaki K · Ito S · Shindo M · Tsuji T · Wake H · Watanabe K · Maeda Y · Enokida T · Tahara M · Yamashita R · Fujisawa T · Nomura M · Kawazoe A · Goto K · Doi T · Shitara K · Mano H · Sekiguchi Y · Nakamura S · Benno Y · Nishikawa H last · Hadi DK first · Baines KJ · Jabbarizadeh B · Miller WH · Jamal R · Ernst S · Logan D · Belanger K · Esfahani K · Elkrief A · Parvathy SN · Silverman MS · Routy B · Maleki Vareki S · Lenehan JG last · Ghozlane A first · Thirion F · Plaza Oñate F · Gauthier F · Le Chatelier E · Annamalé A · Almeida M · Ehrlich SD · Pons N last · Orletskaia VA first · Olekhnovich EI last
Downloads
PRJNA928744.csv.gz (3.2 KB) · parquet (74.5 KB) — all 71 samples, every field with route and confidence · PRJNA928744_determinations.csv.gz (6.5 KB) — 879 evidence rows