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PRJNA929645 SRP421040

Gut microbiota in UC patients undergoing FMT, Nanjing, China

Microbiota Genome sequencing

32 samples from Nanjing, China, from patients with inflammatory bowel disease (ulcerative colitis), studying gut microbiota abundance before and after fecal microbiota transplantation (FMT) in an intervention design. machine-written summary

32 samples · 32 runs · Age unknown · 2024 · 11.48 Gbp / sample

Sources
✓Sequence archive 64–Abstract / description–Full text–Supplementary tables–External resources–User contribution–Human verified

Checked this project's papers and archive records yourself? Mark it human-verified.

what each source gave
✓ Sequence archive64 sample values (health condition, health condition detail)
– Abstract / descriptionno abstract or project description
– Full textno open-access full text linked
– Supplementary tablesno supplementary tables available (or none open access)
– External resourcesnot covered by the external resources we ingest
– User contributionnothing uploaded yet
– Human verifiednot yet checked by a person · I checked this project — verify it
Interventions

intervention — arms not resolved

Faecal microbiota transplantation design single_arm_before_after · population ulcerative_colitis · Washed microbiota transplantation (WMT) in UC patients

“UC patients with or without WMT” — bioproject.description (study level; per-sample arms are in the intervention column below)

Samples at a glance
Age
unknown32
Condition
ulcerative_colitis32
Body site
primary32
Sequencing depth per sample · this project all projects
all projects, 0–0.03 Gbp: 25,080 samples (4 %)all projects, 0.03–0.1 Gbp: 17,066 samples (3 %)all projects, 0.1–0.3 Gbp: 29,179 samples (5 %)all projects, 0.3–1 Gbp: 47,044 samples (8 %)all projects, 1–3 Gbp: 124,983 samples (22 %)all projects, 3–10 Gbp: 253,728 samples (44 %)this project, 3–10 Gbp: 4 samplesall projects, 10–30 Gbp: 72,533 samples (13 %)this project, 10–30 Gbp: 28 samplesall projects, 30–100 Gbp: 3,024 samples (1 %)all projects, 100–300 Gbp: 106 samples (0 %)all projects, ≥ 300 Gbp: 9 samples (0 %)project median 11.48 Gbp per sample0130Gbp
Community composition
Faecalibacterium 17.6%Bifidobacterium 11.9%Phocaeicola 10.9%Prevotella 7.7%Bacteroides 6.9%Blautia_A 6.3%Roseburia 3.1%Collinsella 2.6%Blautia 2.4%Escherichia 1.5%Anaerostipes 1.3%Lachnospira 1.1%not assigned to a genus 3.5%other genera 23.1%

Genera, mean relative abundance over 1 profiled gut samples of this project (Sandpiper SingleM profiles, GTDB R232; low-depth and non-metagenome profiles left out).

Field coverage
fieldsamplescoverageR1R2R3R4
age_category0 0%
country0 0%
health_condition32 100%32
subject_id0 0%
health_condition_detail32 100%32
Details
Archive description

Gut microbiota genomic DNA was extracted from UC patients with or without WMT, and observed the abundance of gut microbiota before and after treatment

How this project entered the registry

Classified as human gut / stool shotgun DNA metagenome of age unknown by sonnet_x2 with confidence 0.78. Assay shotgun_dna.

  • host run.host_tax_id · “9606”
  • body site study.description · “Gut microbiota genomic DNA was extracted”
Evidence examples (one per field × route)
samplefieldvaluerouteconf.sourcequote
SRR23343192health_conditionulcerative_colitisR10.70sample.name_rule:experiment_titleexperiment_title: Illumina NovaSeq 6000 sequencing: metagenome-Seqofgutmicrobiota:UCpatients
SRR23343192health_condition_detailIllumina NovaSeq 6000 sequencing: metagenome-Seqofgutmicrobiota:UCpatientsR10.70sample.name_rule:experiment_titleexperiment_title: Illumina NovaSeq 6000 sequencing: metagenome-Seqofgutmicrobiota:UCpatients
Sequencing

32 runs over 32 samples · mean 11.96 Gbp per run (median 11.48 Gbp, total 382.88 Gbp; 32 runs with a base count) · layout PAIRED 32 · platform ILLUMINA 32 · models Illumina NovaSeq 6000 (32) · 97% of runs with a Sandpiper profile · first public 2024-03-14

Authors (0)

No author record.

Downloads

PRJNA929645.csv.gz (1.6 KB) · parquet (69.6 KB) — all 32 samples, every field with route and confidence · PRJNA929645_determinations.csv.gz (843 B) — 64 evidence rows

Samples (32 · every field this project has values for · hover a cell for its source)
Loading the sample table…