Gut microbiome response to plant-based AWE diet, Singapore
Shotgun metagenomic study of 30 stool samples from 10 healthy adults in Singapore before and during a 21-day plant-based (AWE) diet intervention to assess changes in gut microbiome species and functional pathways. machine-written summary
30 samples · 30 runs · SG · Age unknown · 2023 · 7.20 Gbp / sample
Checked this project's papers and archive records yourself? Mark it human-verified.
what each source gave
| ✓ Sequence archive | 240 sample values (age, bmi, collection date, country, intervention, sex, subject id, timepoint) |
| ○ Abstract / description | read; no usable statement |
| ✓ Full text | 90 sample values (antibiotic exposure, health condition, health condition detail) · PMC10061721 |
| ○ Supplementary tables | files read; no table could be matched to the samples |
| – External resources | not covered by the external resources we ingest |
| – User contribution | nothing uploaded yet |
| – Human verified | not yet checked by a person · I checked this project — verify it |
intervention · arms resolved
10 people have samples in more than one arm.
Controlled / prescribed diet or feeding regimen design single_arm_before_after · population healthy_control · 21-day plant-based 'AWE' diet (OsomeFood Clean Label meals), 5 days/week
“consumed OsomeFood meals for five consecutive weekday lunches and dinners” — abstract:36997838 (study level; per-sample arms are in the intervention column below)
longitudinal — not resolved
10 of 10 people sampled more than once (30 samples; median 3, up to 3 per person). Schedule stated by the source: “Stool samples collected on day 0 (D0/D1), day 7 (D7), and day 21 (D21) of the 21-day intervention, per subject (timepoints T1, T2, T3).” (time origin: intervention/treatment start); stated follow-up 21 d.
No Sandpiper profile for this project's samples yet.
| field | samples | coverage | R1 | R2 | R3 | R4 |
|---|---|---|---|---|---|---|
| age_category | 30 | 100% | ||||
| country | 30 | 100% | 30 | |||
| health_condition | 30 | 100% | 30 | |||
| subject_id | 30 | 100% | 30 | |||
| age_at_collection_days | 30 | 100% | 30 | |||
| antibiotic_exposure | 30 | 100% | 30 | |||
| bmi | 30 | 100% | 30 | |||
| collection_date | 30 | 100% | 30 | |||
| health_condition_detail | 30 | 100% | 30 | |||
| intervention | 30 | 100% | 30 | |||
| sex | 30 | 100% | 30 | |||
| timepoint_label | 30 | 100% | 30 |
- Effects of OsomeFood Clean Label plant-based meals on the gut microbiome. 2023 PMID 36997838 PMC10061721 own_data
Archive description
Effect of AWE diet on the gut microbiome following a 21-day intervention period
How this project entered the registry
Classified as human gut / stool shotgun DNA metagenome of adult by curator_audit with confidence 0.50. Assay shotgun_dna.
- host runs.scientific_name · “human gut metagenome (30)”
- host papers[0].abstract · “species and functional pathway annotations were analyzed by shotgun sequencing”
- body site runs.scientific_name · “human gut metagenome (30)”
- body site papers[0].abstract · “species and functional pathway annotations were analyzed by shotgun sequencing”
- life stage sample.attr.age · “38 (n=30/30)”
Evidence examples (one per field × route)
| sample | field | value | route | conf. | source | quote |
|---|---|---|---|---|---|---|
| SAMN33462946 | age_at_collection_days | 14234.0 | R1 | 0.85 | sample.attr.age | 39 |
| SAMN33462958 | age_at_collection_days | 13879.5 | R1 | 0.85 | biosample.attribute:age | age: 38 |
| SAMN33462946 | antibiotic_exposure | no | R3 | 0.70 | paper.body.methods | Excluded participants were those using oral antibiotics, antifungal and/or antiviral treatments within the prior 3 months |
| SAMN33462946 | bmi | 20.28 | R1 | 0.85 | biosample.attribute:body_mass_index | body_mass_index: 20.2847985719501 |
| SAMN33462946 | collection_date | 2022-07 | R1 | 0.85 | biosample.attribute:collection_date | collection_date: 2022-07 |
| SAMN33462946 | country | SG | R1 | 0.90 | sample.attr.geo_loc_name | Singapore |
| SAMN33462946 | health_condition | healthy_control | R3 | 0.70 | paper.body.methods | ten healthy participants consumed OsomeFood meals |
| SAMN33462946 | health_condition_detail | healthy participants | R3 | 0.70 | paper.body.methods | ten healthy participants consumed OsomeFood meals |
| SAMN33462946 | intervention | no_intervention | R1 | 0.70 | biosample.attribute:timepoint+paper.body.Methods | timepoint: T1 |
| SAMN33462946 | sex | female | R1 | 0.90 | sample.attr.sex | female |
| SAMN33462946 | subject_id | AWE001 | R1 | 0.85 | biosample.attribute:subject | subject: AWE001 |
| SAMN33462946 | timepoint_label | T1 | R1 | 0.85 | biosample.attribute:timepoint | timepoint: T1 |
Sequencing
30 runs over 30 samples · mean 7.14 Gbp per run (median 7.20 Gbp, total 214.20 Gbp; 30 runs with a base count) · layout PAIRED 30 · platform ILLUMINA 30 · models Illumina NovaSeq 6000 (30) · 90% of runs with a Sandpiper profile · first public 2023-03-01
Authors (0)
No author record.
Downloads
PRJNA939268.csv.gz (1.6 KB) · parquet (71.4 KB) — all 30 samples, every field with route and confidence · PRJNA939268_determinations.csv.gz (3.2 KB) — 330 evidence rows