Diet-Gut Microbiome Crossover Trial, Orlando, USA
44 fecal samples from healthy adults in Orlando, USA, in a randomized crossover feeding trial comparing a western diet and a high-fiber diet, examining methanogens, hydrogenotrophs, and host energy balance. machine-written summary
44 samples · 44 runs · US · Age unknown · 2023 · 2.72 Gbp / sample · cohort Host-diet-gut microbiome interactions influence hu
One study deposited as 2 BioProjects (125 catalog samples together): PRJNA913183 · PRJNA947193 · show together
Checked this project's papers and archive records yourself? Mark it human-verified.
what each source gave
| ✓ Sequence archive | 264 sample values (collection date, country, latitude, location locality, location region, longitude) |
| ✓ Abstract / description | 44 sample values (health condition) |
| ○ Full text | read; no usable statement or table · PMC10232526, PMC11949708, PMC12156016 |
| ○ Supplementary tables | files read; no table could be matched to the samples |
| – External resources | not covered by the external resources we ingest |
| – User contribution | nothing uploaded yet |
| – Human verified | not yet checked by a person · I checked this project — verify it |
intervention — arms not resolved
Controlled / prescribed diet or feeding regimen design crossover · population healthy_control · Randomized crossover controlled feeding: Western diet vs Microbiome Enhancer Diet
“controlled feeding study with a randomized crossover design” — abstract:37258525 (study level; per-sample arms are in the intervention column below)
Genera, mean relative abundance over 36 profiled gut samples of this project (Sandpiper SingleM profiles, GTDB R232; low-depth and non-metagenome profiles left out).
| field | samples | coverage | R1 | R2 | R3 | R4 |
|---|---|---|---|---|---|---|
| age_category | 0 | 0% | ||||
| country | 44 | 100% | 44 | |||
| health_condition | 44 | 100% | 44 | |||
| subject_id | 0 | 0% | ||||
| collection_date | 44 | 100% | 44 | |||
| detailed_location | 44 | 100% | ||||
| latitude | 44 | 100% | 44 | |||
| location_locality | 44 | 100% | 44 | |||
| location_region | 44 | 100% | 44 | |||
| longitude | 44 | 100% | 44 |
- Host-diet-gut microbiome interactions influence human energy balance: a randomized clinical trial. 2023 PMID 37258525 PMC10232526 unsure
- Metagenomic estimation of dietary intake from human stool. 2025 PMID 39966520 PMC11949708 unsure
- Methanogenesis associated with altered microbial production of short-chain fatty acids and human-host metabolizable energy. 2025 PMID 40403748 PMC12156016 unsure
Archive description
Quantify microbial contribution to host energy balance. Processed sequence files.
How this project entered the registry
Classified as human gut / stool shotgun DNA metagenome of age unknown by sonnet_x2 with confidence 0.72. Assay shotgun_dna.
- host run.host_tax_id · “9606”
- body site run.scientific_name · “feces metagenome”
Evidence examples (one per field × route)
| sample | field | value | route | conf. | source | quote |
|---|---|---|---|---|---|---|
| SAMN33799461 | collection_date | 2020 | R1 | 0.90 | biosample.attribute:collection_date | 2020 |
| SAMN33799461 | country | US | R1 | 0.90 | sample.attr.geo_loc_name | USA: Orlando |
| SAMN33799461 | health_condition | healthy_control | R4 | 0.50 | paper.abstract | young, healthy, weight stable males and females |
| SAMN33799461 | latitude | 28.5384 | R1 | 0.90 | biosample.attribute:lat_lon | 28.5384 N 81.3789 W |
| SAMN33799461 | location_locality | Orlando | R1 | 0.85 | biosample.attribute:geo_loc_name | USA: Orlando |
| SAMN33799461 | location_region | Florida | R1 | 0.60 | biosample.attribute:lat_lon | 28.5384 N 81.3789 W |
| SAMN33799461 | longitude | -81.3789 | R1 | 0.90 | biosample.attribute:lat_lon | 28.5384 N 81.3789 W |
Sequencing
44 runs over 44 samples · mean 2.98 Gbp per run (median 2.72 Gbp, total 131.01 Gbp; 44 runs with a base count) · layout PAIRED 44 · platform ILLUMINA 44 · models Illumina HiSeq 4000 (44) · 91% of runs with a Sandpiper profile · first public 2023-06-05
Authors (16)
Corbin KD first · Carnero EA · Dirks B · Igudesman D · Yi F · Marcus A · Davis TL · Pratley RE · Rittmann BE · Krajmalnik-Brown R · Smith SR last · Diener C first · Holscher HD · Filek K · Moissl-Eichinger C · Gibbons SM last
Downloads
PRJNA947193.csv.gz (1.9 KB) · parquet (71.0 KB) — all 44 samples, every field with route and confidence · PRJNA947193_determinations.csv.gz (2.3 KB) — 308 evidence rows