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PRJNA971196 SRP436865

Gut metagenomes of dairy workers and community controls, US

Healthy Dairy Worker Metagenomics Study

Shotgun metagenomic sequencing of 16 adult gut samples from dairy farm workers and community controls in the United States, comparing microbiome taxonomy, diversity, and antibiotic resistance gene prevalence. machine-written summary

16 samples · 16 runs · US · Age unknown · 2023 · 7.14 Gbp / sample

Sources
✓Sequence archive 44✓Abstract / description 16✓Full text 16○Supplementary tables–External resources–User contribution–Human verified

Checked this project's papers and archive records yourself? Mark it human-verified.

what each source gave
✓ Sequence archive44 sample values (collection date, country, health condition, health condition detail)
✓ Abstract / description16 sample values (location region)
✓ Full text16 sample values (subject id) · PMC11626760
○ Supplementary tablesfiles read; no table could be matched to the samples
– External resourcesnot covered by the external resources we ingest
– User contributionnothing uploaded yet
– Human verifiednot yet checked by a person · I checked this project — verify it
Samples at a glance
1 country
Age
adult16
Condition
healthy_control6
Country
US16
Body site
primary16
Sequencing depth per sample · this project all projects
all projects, 0–0.03 Gbp: 25,080 samples (4 %)all projects, 0.03–0.1 Gbp: 17,066 samples (3 %)all projects, 0.1–0.3 Gbp: 29,179 samples (5 %)all projects, 0.3–1 Gbp: 47,044 samples (8 %)all projects, 1–3 Gbp: 124,983 samples (22 %)all projects, 3–10 Gbp: 253,728 samples (44 %)this project, 3–10 Gbp: 15 samplesall projects, 10–30 Gbp: 72,533 samples (13 %)this project, 10–30 Gbp: 1 samplesall projects, 30–100 Gbp: 3,024 samples (1 %)all projects, 100–300 Gbp: 106 samples (0 %)all projects, ≥ 300 Gbp: 9 samples (0 %)project median 7.14 Gbp per sample0130Gbp
Community composition

No Sandpiper profile for this project's samples yet.

Field coverage
fieldsamplescoverageR1R2R3R4
age_category16 100%
country16 100%16
health_condition6 38%6
subject_id16 100%16
collection_date16 100%16
detailed_location16 100%
location_region16 100%16
health_condition_detail6 38%6
Papers (1)
  • A cross-sectional comparison of gut metagenomes between dairy workers and community controls. 2024 PMID 39033279 PMC11626760 cites_accession
Details
Archive description

<![CDATA[The project is interested in characterizing the microbiome and resistome of dairy workers,</p><p>whose exposure to the livestock farming environment places them at risk for</p><p>facilitating community transmission of antibiotic resistant genes and emerging</p><p>zoonotic diseases. Using shotgun metagenomics sequencing, we investigated differences in the taxonomy,</p><p>diversity and gene presence of the human gut microbiome of 10 dairy farm</p><p>workers and 6 community controls, with a focus on antibiotic resistance genes</p><p>and virulence factors in the occupationally-exposed cohort.]]>

How this project entered the registry

Classified as human gut / stool shotgun DNA metagenome of adult by sonnet_x2 with confidence 0.75. Assay shotgun_dna.

  • host run.host_tax_id · “9606”
  • body site study.description · “human gut microbiome of 10 dairy farm”
  • life stage study.description · “dairy farm workers and 6 community controls”
Evidence examples (one per field × route)
samplefieldvaluerouteconf.sourcequote
SAMN35024728collection_date2018-02-15R10.85biosample.attribute:collection_datecollection_date: 2018-02-15
SAMN35024728countryUSR10.85biosample.attribute:geo_loc_namegeo_loc_name: USA: Yakima Valley, WA
SAMN35024728health_conditionhealthy_controlR10.70sample.name_rule:titleC02-S104
SAMN35024728health_condition_detailC02-S104R10.70sample.name_rule:titleC02-S104
SAMN35024728location_regionUnited StatesR40.50paper.abstracta cohort of dairy workers in the United States
SAMN35024728subject_idSAMN35024728R30.75design.one_sample_per_subject:fulltext16 fecal samples (the maximum possible with budget constraints) taken from the Healthy Dairy Worker study cohort. These samples came from 10 dairy workers and
Sequencing

16 runs over 16 samples · mean 7.33 Gbp per run (median 7.14 Gbp, total 117.31 Gbp; 16 runs with a base count) · layout PAIRED 16 · platform ILLUMINA 16 · models Illumina HiSeq 2500 (16) · 0% of runs with a Sandpiper profile · first public 2023-06-22 – 2023-06-23

Authors (0)

No author record.

Downloads

PRJNA971196.csv.gz (1.3 KB) · parquet (70.1 KB) — all 16 samples, every field with route and confidence · PRJNA971196_determinations.csv.gz (1.5 KB) — 76 evidence rows

Samples (16 · every field this project has values for · hover a cell for its source)
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