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PRJNA984213 SRP444673

B. longum persistence in healthy Italian elderly gut

Evaluation of the persistence of Bifidobacterium longum subsp. longum in the human gut across the entire life span

19 fecal samples from healthy elderly individuals (over 80 years, average age 84) in Italy, cross-sectional design, examining persistence and abundance of Bifidobacterium longum subsp. longum in the gut. machine-written summary

19 samples · 19 runs · IT · Adult · 2023 · 38.4 Mbp / sample

Sources
✓Sequence archive 95✓Abstract / description 38–Full text–Supplementary tables–External resources–User contribution–Human verified

Checked this project's papers and archive records yourself? Mark it human-verified.

what each source gave
✓ Sequence archive95 sample values (collection date, country, latitude, location region, longitude)
✓ Abstract / description38 sample values (health condition, subject id)
– Full textno open-access full text linked
– Supplementary tablesno supplementary tables available (or none open access)
– External resourcesnot covered by the external resources we ingest
– User contributionnothing uploaded yet
– Human verifiednot yet checked by a person · I checked this project — verify it
Samples at a glance
1 sampling locations · 1 country
Age
elderly19
Condition
healthy_control19
Country
IT19
Body site
primary19
Sequencing depth per sample · this project all projects
all projects, 0–0.03 Gbp: 25,080 samples (4 %)this project, 0–0.03 Gbp: 7 samplesall projects, 0.03–0.1 Gbp: 17,066 samples (3 %)this project, 0.03–0.1 Gbp: 12 samplesall projects, 0.1–0.3 Gbp: 29,179 samples (5 %)all projects, 0.3–1 Gbp: 47,044 samples (8 %)all projects, 1–3 Gbp: 124,983 samples (22 %)all projects, 3–10 Gbp: 253,728 samples (44 %)all projects, 10–30 Gbp: 72,533 samples (13 %)all projects, 30–100 Gbp: 3,024 samples (1 %)all projects, 100–300 Gbp: 106 samples (0 %)all projects, ≥ 300 Gbp: 9 samples (0 %)project median 0.04 Gbp per sample0130Gbp
Community composition

No Sandpiper profile for this project's samples yet.

Field coverage
fieldsamplescoverageR1R2R3R4
age_category19 100%
country19 100%19
health_condition19 100%19
subject_id19 100%19
collection_date19 100%19
detailed_location19 100%
latitude19 100%19
location_region19 100%19
longitude19 100%19
Details
Archive description

19 fecal samples were collected from healthy elderly with more than 80 years (average age = 84), aiming to discover additional B. longum strains for further in vitro experimentation. Thus, shallow shotgun metagenomic sequencing was performed to corroborate the abundance of B. longum species that populate healthy elderly surveyed in this study.

How this project entered the registry

Classified as human gut / stool shotgun DNA metagenome of adult and elderly by deterministic_prior with confidence 0.85. Assay shotgun_dna.

  • host external_curation.infant_catalog · “infant triage reason_code age_adult_only”
  • body site study.title · “in the human gut across the entire”
  • body site study.description · “19 fecal samples were collected”
  • life stage external_curation.infant_catalog · “infant triage reason_code age_adult_only”
  • life stage study.description · “collected from healthy elderly with more than”
Evidence examples (one per field × route)
samplefieldvaluerouteconf.sourcequote
SAMN35748290collection_date2022-05-16R10.90biosample.attribute:collection_date2022-05-16
SAMN35748290countryITR10.90sample.attr.geo_loc_nameItaly
SAMN35748290health_conditionhealthy_controlR40.50study.descriptionhealthy elderly
SAMN35748290latitude44.7680R10.90biosample.attribute:lat_lon44.76797887 N 10.31372768 E
SAMN35748290location_regionEmilia-RomagnaR10.60biosample.attribute:lat_lon44.76797887 N 10.31372768 E
SAMN35748290longitude10.3137R10.90biosample.attribute:lat_lon44.76797887 N 10.31372768 E
SAMN35748290subject_idSAMN35748290R40.60design.one_sample_per_subject:description19 fecal samples were collected from healthy elderly with more than 80 years (average age = 84)
Sequencing

19 runs over 19 samples · mean 35.7 Mbp per run (median 38.4 Mbp, total 0.68 Gbp; 19 runs with a base count) · layout PAIRED 19 · platform ILLUMINA 19 · models Illumina MiSeq (19) · 0% of runs with a Sandpiper profile · first public 2023-07-02

Authors (0)

No author record.

Downloads

PRJNA984213.csv.gz (1.4 KB) · parquet (70.6 KB) — all 19 samples, every field with route and confidence · PRJNA984213_determinations.csv.gz (1.6 KB) — 133 evidence rows

Samples (19 · every field this project has values for · hover a cell for its source)
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