Home › Studies › PRJNA995911
PRJNA995911 SRP450121

Fecal metagenomics of apple pomace pectin metabolism, Spain

Fecal microbiota cooperative metabolism of pectins derived from apple pomace: a functional metagenomic study

Shotgun metagenomic study of 41 fecal samples from Asturias, Spain, examining cooperative gut microbial metabolism of pectins derived from apple pomace as a prebiotic/fibre intervention. machine-written summary

41 samples · 41 runs · ES · Age unknown · 2023 · 6.89 Gbp / sample

Sources
✓Sequence archive 293○Abstract / description–Full text–Supplementary tables–External resources–User contribution–Human verified

Checked this project's papers and archive records yourself? Mark it human-verified.

what each source gave
✓ Sequence archive293 sample values (collection date, country, health condition, health condition detail, intervention, location region, subject id, timepoint)
○ Abstract / descriptionread; no usable statement
– Full textno open-access full text linked
– Supplementary tablesno supplementary tables available (or none open access)
– External resourcesnot covered by the external resources we ingest
– User contributionnothing uploaded yet
– Human verifiednot yet checked by a person · I checked this project — verify it
Interventions

intervention · arms resolved

EPrebiotic / dietary fibre supplement: 6 people · 35 samplesprebiotic / fibre 6 people · 35 samplesControl / pre-intervention baseline (sample level only): 6 people · 6 samplesno intervention 6 people · 6 samples
exposed vs. not exposed (observational)

6 people have samples in more than one arm.

Prebiotic / dietary fibre supplement design observational_with_procedure · In vitro fecal fermentation with apple pomace-derived pectins

“cooperative metabolism of pectins derived from apple pomace” — study.title (study level; per-sample arms are in the intervention column below)

Samples at a glance
1 country
Age
unknown41
Condition
healthy_control21
crohns_disease20
Country
ES41
Body site
primary41
Sequencing depth per sample · this project all projects
all projects, 0–0.03 Gbp: 25,080 samples (4 %)all projects, 0.03–0.1 Gbp: 17,066 samples (3 %)all projects, 0.1–0.3 Gbp: 29,179 samples (5 %)all projects, 0.3–1 Gbp: 47,044 samples (8 %)all projects, 1–3 Gbp: 124,983 samples (22 %)this project, 1–3 Gbp: 5 samplesall projects, 3–10 Gbp: 253,728 samples (44 %)this project, 3–10 Gbp: 36 samplesall projects, 10–30 Gbp: 72,533 samples (13 %)all projects, 30–100 Gbp: 3,024 samples (1 %)all projects, 100–300 Gbp: 106 samples (0 %)all projects, ≥ 300 Gbp: 9 samples (0 %)project median 6.89 Gbp per sample0130Gbp
Sampling design and relatedness

longitudinal — not resolved

6 of 6 people sampled more than once (41 samples; median 7, up to 7 per person). Schedule stated by the source: “Only a '0h' timepoint_label is given (15% coverage); title indicates in vitro fecal fermentation of apple pomace pectins, so '0h' likely marks fermentation incubation start, not repeated human samplin”.

How longitudinal and relatedness information is derived

Community composition
Blautia_A 7.5%Bifidobacterium 6.9%Prevotella 6.6%Roseburia 5.6%Faecalibacterium 5.2%Bacteroides 3.6%Phocaeicola 3.6%Alistipes 3.2%Collinsella 3.1%Akkermansia 3.1%Ruminococcoides 2.6%Gemmiger 1.8%not assigned to a genus 5.2%other genera 41.9%

Genera, mean relative abundance over 34 profiled gut samples of this project (Sandpiper SingleM profiles, GTDB R232; low-depth and non-metagenome profiles left out).

Field coverage
fieldsamplescoverageR1R2R3R4
age_category0 0%
country41 100%41
health_condition41 100%41
subject_id41 100%41
collection_date41 100%41
detailed_location41 100%
health_condition_detail41 100%41
intervention41 100%41
location_region41 100%41
timepoint_label6 15%6
Papers (1)
  • Fecal microbiota cooperative metabolism of pectins derived from apple pomace: A functional metagenomic study 2023 own_data
Details
Archive description

Shotgun metagenomics sequencing data for the article "Fecal microbiota cooperative metabolism of pectins derived from apple pomace: a functional metagenomic study"

How this project entered the registry

Classified as human gut / stool shotgun DNA metagenome of age unknown by sonnet_x2 with confidence 0.85. Assay shotgun_dna.

  • host run.host_tax_id · “9606”
  • host run.scientific_name · “human gut metagenome(41)”
  • body site run.scientific_name · “human gut metagenome(41)”
Evidence examples (one per field × route)
samplefieldvaluerouteconf.sourcequote
SAMN36510064collection_date2022-03-20R10.90biosample.attribute:collection_date2022-03-20
SAMN36510064countryESR10.90sample.attr.geo_loc_nameSpain: Asturias
SAMN36510064health_conditionhealthy_controlR10.70sample.name_rule:descriptionFecal fermentation Healthy donor 1 - Homogenate - Time: 0h
SAMN36510064health_condition_detailHealthy / control (name token 'Healthy')R10.70sample.name_rule:descriptionFecal fermentation Healthy donor 1 - Homogenate - Time: 0h
SAMN36510064interventionno_interventionR10.70sample.name_rule:descriptionFecal fermentation Healthy donor 1 - Homogenate - Time: 0h
SAMN36510064location_regionAsturiasR10.85biosample.attribute:geo_loc_nameSpain: Asturias
SAMN36510064subject_idHealthy donor 1R10.70sample.name_rule:descriptionFecal fermentation Healthy donor 1 - Homogenate - Time: 0h
SAMN36510064timepoint_label0hR10.70sample.name_rule:descriptionFecal fermentation Healthy donor 1 - Homogenate - Time: 0h
Sequencing

41 runs over 41 samples · mean 6.34 Gbp per run (median 6.89 Gbp, total 259.75 Gbp; 41 runs with a base count) · layout PAIRED 41 · platform ILLUMINA 41 · models Illumina NovaSeq 6000 (41) · 83% of runs with a Sandpiper profile · first public 2023-10-21

Authors (0)

No author record.

Downloads

PRJNA995911.csv.gz (2.0 KB) · parquet (71.3 KB) — all 41 samples, every field with route and confidence · PRJNA995911_determinations.csv.gz (3.0 KB) — 293 evidence rows

Samples (41 · every field this project has values for · hover a cell for its source)
Loading the sample table…