Home › Collections › Protocol benchmarks with mock-community controls

Protocol benchmarks with mock-community controls

Benchmark and reference cohorts · collection id protocol-benchmarks-mock-controls

Human stool studies that sequenced a defined mock community (ZymoBIOMICS or in-house standards) alongside real samples to compare DNA extraction kits, library protocols or 16S versus shotgun profiling — useful as positive controls and for bias estimation.

Open in the sample explorer (577 samples)

5
studies with member samples
577
member samples in this release

Membership rule

StudiesPRJEB38036, PRJEB75510, PRJNA648321, PRJNA911046, PRJNA725047

Selected by the word 'mock' in the ENA study description; the mock-community runs themselves are not human samples and may be classified body_site_class excluded/unknown.

Composition

Age category
unknown294
adult253
child30
Country
DK233
CN162
CH96
FR86
Health condition
healthy_control Healthy / control54
gi_infection_or_diarrhoea GI infection / diarrhoea (incl. C. difficile)24

Member studies (5)

StudyTitleMember samplesFirst public
PRJEB38036Assessment of fecal DNA extraction protocols for metagenomic study2332020
PRJNA72504716s vs shallowshotgun1622021
PRJEB75510Evaluation of DNA extraction kits for long-read shotgun metagenomics using Oxford Nanopore sequencing for rapid taxonomic and antimicrobial resistance detection962024
PRJNA648321Comparison of DNA extraction protocols from stool samples562022
PRJNA911046Multicenter evaluation study of gut microbiome profiling by next-generation sequencing302023

References